RING1 Polyclonal antibody

RING1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 15037-1-AP

Synonyms

Polycomb complex protein RING1, ring finger protein 1, RING1, RING1A, RNF1

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Validation Data Gallery

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  WB analysis of mouse skeletal muscle using 15037-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 15037-1-AP (RING1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of BxPC-3 using 15037-1-AP

  BxPC-3 cells were subjected to SDS PAGE followed by western blot with 15037-1-AP (RING1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 15037-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 15037-1-AP (RING1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human prostate cancer using 15037-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 15037-1-AP (RING1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human prostate cancer using 15037-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 15037-1-AP (RING1 antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: mouse skeletal muscle tissue, BxPC-3 cells, PC-3 cells
• Positive IHC detected in: human prostate cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

15037-1-AP targets RING1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: RING1 fusion protein Ag6763
• Full Name: ring finger protein 1
• Calculated Molecular Weight: 42 kDa
• Observed Molecular Weight: 50 kDa
• GenBank Accession Number: BC002922
• Gene Symbol: RING1
• Gene ID (NCBI): 6015
• RRID: AB_2177964
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Ring1, also named as Ring1a, constitutes one of the E3 ubiquitin-protein ligases that mediate monoubiquitination of 'Lys-119' of histone H2A, thereby playing a central role in histone code and gene regulation. H2A 'Lys-119' ubiquitination gives a specific tag for epigenetic transcriptional repression and participates in X chromosome inactivation of female mammals. It's an essential component of a Polycomb group (PcG) multiprotein PRC1-like complex, a complex class required to maintain the transcriptionally repressive state of many genes, including Hox genes, throughout development. PcG PRC1 complex acts via chromatin remodeling and modification of histones, rendering chromatin heritably changed in its expressibility. Compared to RNF2/RING2, it does not have the main E3 ubiquitin ligase activity on histone H2A, and it may rather act as a modulator of RNF2/RING2 activity.