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AXL Polyklonaler Antikörper

AXL Polyklonal Antikörper für WB, IP, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human und mehr (1)

Anwendung

WB, IP, IF, IHC, CoIP, ELISA

Konjugation

Unkonjugiert

Kat-Nr. : 13196-1-AP

Synonyme

AXL, AXL oncogene, AXL receptor tyrosine kinase, JTK11, UFO



Geprüfte Anwendungen

Erfolgreiche Detektion in WBDU 145-Zellen, HeLa-Zellen, HepG2-Zellen, HT-1080.Zellen, humanes Lebergewebe
Erfolgreiche IPHeLa-Zellen

Empfohlene Verdünnung

AnwendungVerdünnung
Western Blot (WB)WB : 1:1000-1:8000
Immunpräzipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Produktinformation

13196-1-AP bindet in WB, IP, IF, IHC, CoIP, ELISA AXL und zeigt Reaktivität mit human

Getestete Reaktivität human
In Publikationen genannte Reaktivitäthuman, Ratte
Wirt / Isotyp Kaninchen / IgG
Klonalität Polyklonal
Typ Antikörper
Immunogen AXL fusion protein Ag3898
Vollständiger Name AXL receptor tyrosine kinase
Berechnetes Molekulargewicht 894 aa, 98 kDa
Beobachtetes Molekulargewicht 140 kDa
GenBank-ZugangsnummerBC032229
Gene symbol AXL
Gene ID (NCBI) 558
Konjugation Unkonjugiert
Form Liquid
Reinigungsmethode Antigen-Affinitätsreinigung
Lagerungspuffer PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
LagerungsbedingungenBei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

Axl, also known as Ufo or Ark, is a receptor tyrosine kinase of the TAM (Tyro3, Axl and Mer) family. Axl is a single-pass type I membrane protein containing two fibronectin type-III domains, two Ig-like C2-type domains and one protein kinase domain. Axl mRNA is widely expressed with highest levels in the heart and skeletal muscle. Gas6 has been identified as a ligand for human Axl. Gas6/Axl signaling plays a role in cell survival, cell proliferation, migration and differentiation. It has been reported that 110-120 kDa non-glycosylated AXL protein can be detected(PMID: 31171001). Endogenous Axl is auto-phosphorylated and reacted with a tyrosine-phosphorylated protein of 140 kDa(PMID: 12730092).

Protokolle

Produktspezifische Protokolle
WB protocol for AXL antibody 13196-1-APProtokoll herunterladen
IP protocol for AXL antibody 13196-1-APProtokoll herunterladen
Standard-Protokolle
Klicken Sie hier, um unsere Standardprotokolle anzuzeigen

Publikationen

SpeciesApplicationTitle
humanWB

Int J Cancer

Therapeutic Activity of DCC-2036, a Novel Tyrosine Kinase Inhibitor, against Triple-Negative Breast Cancer Patient-Derived Xenografts by Targeting AXL/MET.

Authors - Yingying Shen
ratWB

Int J Mol Sci

Embryonic-Derived Myb- Macrophages Enhance Bacterial Clearance and Improve Survival in Rat Sepsis.

Authors - Mirjana Jerkic
humanWB

Int J Mol Sci

Upregulation of miR-34a-5p, miR-20a-3p and miR-29a-3p by Onconase in A375 Melanoma Cells Correlates with the Downregulation of Specific Onco-Proteins.

Authors - Elisa De Tomi
FC

Bioorg Chem

Non-volatile acylphloroglucinol components from Eucalyptus robusta inhibit Zika virus by impairing RdRp activity of NS5.

Authors - Zhai-Wen Yao
humanWB, IHC,IF

Cancer Gene Ther

Targeting LRIG2 overcomes resistance to EGFR inhibitor in glioblastoma by modulating GAS6/AXL/SRC signaling.

Authors - Minhai Dong
humanWB

iScience

SUFU suppresses ferroptosis sensitivity in breast cancer cells via Hippo/YAP pathway.

Authors - Kun Fang

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

Marta (Verified Customer) (05-30-2022)

The antibody works well. Non-specific bands are not detected.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:2000
  • Cell Tissue Type: A375 melanoma cell line