ARC/ARG3.1 Polyklonaler Antikörper

• Featured Product
• KD/KO Validated

ARC/ARG3.1 Polyklonal Antikörper für IHC, IP, WB, ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human, Maus, Ratte

Anwendung

WB, IP, IHC, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

21

Rezensionen (2)

Kat-Nr. : 16290-1-AP

Synonyme

ARC, ARC/ARG3.1, Arg3.1, KIAA0278

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Galerie der Validierungsdaten

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  WB analysis using 16290-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16290-1-AP (ARC/ARG3.1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SH-SY5Y using 16290-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 16290-1-AP (ARC/ARG3.1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of SH-SY5Y using 16290-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 16290-1-AP (ARC/ARG3.1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat heart using 16290-1-AP

  rat heart tissue were subjected to SDS PAGE followed by western blot with 16290-1-AP (ARC/ARG3.1 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 16290-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 16290-1-AP (ARC/ARG3.1 antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 16290-1-AP

  IP Result of anti-ARC/ARG3.1 (IP:16290-1-AP, 4ug; Detection:16290-1-AP 1:300) with mouse brain tissue lysate 4000ug.

• 

  IHC staining of mouse brain using 16290-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 16290-1-AP (ARC/ARG3.1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human brain using 16290-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 16290-1-AP (ARC/ARG3.1 antibody) at dilution of 1:100 (under 40x lens).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: SH-SY5Y-Zellen, Maushirngewebe, Rattenherzgewebe, Rattenhirngewebe
• Erfolgreiche IP: Maushirngewebe
• Erfolgreiche Detektion in IHC: Maushirngewebe, humanes Hirngewebe; Hinweis: Antigendemaskierung mit TE-Puffer pH 9,0 empfohlen. (*) Wahlweise kann die Antigendemaskierung auch mit Citratpuffer pH 6,0 erfolgen.

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2000
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunhistochemie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• KD/KO: See 3 publications below
• WB: See 18 publications below
• IHC: See 7 publications below
• IF: See 5 publications below
• IP: See 1 publications below

Produktinformation

16290-1-AP bindet in WB, IP, IHC, IF, ELISA ARC/ARG3.1 und zeigt Reaktivität mit human, Maus, Ratten

• Getestete Reaktivität: human, Maus, Ratte
• In Publikationen genannte Reaktivität: human, Maus, Ratte
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: ARC/ARG3.1 fusion protein Ag9445
• Vollständiger Name: activity-regulated cytoskeleton-associated protein
• Berechnetes Molekulargewicht: 396 aa, 45 kDa
• Beobachtetes Molekulargewicht: 45-50 kDa
• GenBank-Zugangsnummer: BC012321
• Gene symbol: ARC
• Gene ID (NCBI): 23237
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

ARC, also named as KIAA0278 and Arg3.1, mediates endocytosis of neuronal AMPA-type glutamate receptors (AMPARs). It is required for consolidation of synaptic plasticity as well as formation of long-term memory. ARC plays a role in the regulation of cell morphology and cytoskeletal organization. It is required in the stress fiber dynamics and cell migration.

Publikationen

Species	Application	Title
human	WB	Mol Cell; PQBP1 promotes translational elongation and regulates hippocampal mGluR-LTD by suppressing eEF2 phosphorylation.; Authors - Yuqian Shen
mouse	WB	Neuron; Astrocytic ApoE reprograms neuronal cholesterol metabolism and histone-acetylation-mediated memory.; Authors - Xiaohui Li
rat	WB,IHC	Aging (Albany NY); EGB761 ameliorates chronic cerebral hypoperfusion-induced cognitive dysfunction and synaptic plasticity impairment.; Authors - Zhao-Hui Yao
human	WB	Aging (Albany NY); Repressor element-1 silencing transcription factor regulates glutamate receptors and immediate early genes to affect synaptic plasticity.; Authors - Chenhaoyi Xu
human	WB	Neurobiol Dis; Prions induce an early Arc response and a subsequent reduction in mGluR5 in the hippocampus.; Authors - Daniel Ojeda-Juarez
mouse	IF,WB,IP	Front Mol Neurosci; Activity-Dependent Arc Expression and Homeostatic Synaptic Plasticity Are Altered in Neurons from a Mouse Model of Angelman Syndrome.; Authors - Elissa D Pastuzyn

Rezensionen

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Georgiana (Verified Customer) (01-04-2023)

For the image, magenta is Arc and cyan is GFAP

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: Mouse brain tissue, 20um slices

ARC/ARG3.1 Antibody Immunofluorescence validation (1:500 dilution) in Mouse brain tissue, 20um slices (Cat no:16290-1-AP)

FH

Xin (Verified Customer) (01-23-2022)

Although there are some non specific bands, there is a major band at the expected 50 kD.

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: mouse cortex lysate

ARC/ARG3.1 Antibody Western Blot validation (1:2000 dilution) in mouse cortex lysate (Cat no:16290-1-AP)

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