APAF1 Polyclonal antibody

• Featured Product
• KD/KO Validated

APAF1 Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat and More (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

44

Cat no : 21710-1-AP

Synonyms

APAF 1, APAF1, CED4, KIAA0413

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Validation Data Gallery

• 

  WB analysis of HEK-293 using 21710-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 21710-1-AP (APAF1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of COLO 320 using 21710-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 21710-1-AP (APAF1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 21710-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 21710-1-AP (APAF1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HEK-293 using 21710-1-AP

  IP result of anti-APAF1 (IP:21710-1-AP, 4ug; Detection:21710-1-AP 1:500) with HEK-293 cells lysate 2000ug.

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  IHC staining of human heart using 21710-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 21710-1-AP (APAF1 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human heart using 21710-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 21710-1-AP (APAF1 Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human heart using 21710-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 21710-1-AP (APAF1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 21710-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 21710-1-AP (APAF1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of A549 using 21710-1-AP

  Immunofluorescent analysis of A549 cells using 21710-1-AP (APAF1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: HEK-293 cells, A549 cells, COLO 320 cells
• Positive IP detected in: HEK-293 cells
• Positive IHC detected in: human heart tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: A549 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 43 publications below
• IHC: See 3 publications below
• IF: See 2 publications below

Product Information

The immunogen of 21710-1-AP is APAF1 Fusion Protein expressed in E. coli.

• Tested Reactivity: human, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: APAF1 fusion protein Ag16336
• Full Name: apoptotic peptidase activating factor 1
• Calculated Molecular Weight: 1248 aa, 142 kDa
• Observed Molecular Weight: 130-142 kDa
• GenBank Accession Number: BC136532
• Gene Symbol: APAF1
• Gene ID (NCBI): 317
• RRID: AB_10859252
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

APAF1, also named as Apoptotic protease-activating factor 1 or KIAA0413, is a 1248 amino acid protein, which contains one CARD domain, contains one NB-ARC domain and Contains fifteen WD repeats. APAF1 localizes in the cytoplasm and is widely expressed in many tissues. Highest levels of expression is in adult spleen, peripheral blood leukocytes, in fetal brain, kidney and lung. Oligomeric APAF1 mediates the cytochrome c-dependent autocatalytic activation of pro-caspase-9 (Apaf-3), leading to the activation of caspase-3 and apoptosis.

Publications

Species	Application	Title
mouse	WB	Theranostics; Pro-Inflammatory CXCR3 Impairs Mitochondrial Function in Experimental Non-Alcoholic Steatohepatitis.; Authors - Jinghua Du
human	WB	Oxid Med Cell Longev; Apoptotic Protease Activating Factor-1 Inhibitor Mitigates Myocardial Ischemia Injury via Disturbing Procaspase-9 Recruitment by Apaf-1.; Authors - Ying Wang
human	WB,IHC,IF	Mol Ther Nucleic Acids; LNC473 Regulating APAF1 IRES-Dependent Translation via Competitive Sponging miR574 and miR15b: Implications in Colorectal Cancer.; Authors - Huizhe Wu
rat	WB	Cell Biol Toxicol; Inhibition of ER stress attenuates kidney injury and apoptosis induced by 3-MCPD via regulating mitochondrial fission/fusion and Ca2+ homeostasis.; Authors - Yujie Zhong
human	WB	Front Cell Dev Biol; Antagonizing CDK8 Sensitizes Colorectal Cancer to Radiation Through Potentiating the Transcription of e2f1 Target Gene apaf1.; Authors - Bin Chen
human	WB	Cells; FUT2 Facilitates Autophagy and Suppresses Apoptosis via p53 and JNK Signaling in Lung Adenocarcinoma Cells; Authors - Yuqi Zhang

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