IFNGR2 Polyclonal antibody

IFNGR2 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 10266-1-AP

Synonyms

AF 1, IFGR2, IFN gamma R2, IFN gamma receptor 2, IFNGR2, IFNGT1, Interferon gamma receptor 2, Interferon gamma transducer 1



Tested Applications

Positive WB detected inA549 cells, HeLa cells, HepG2 cells
Positive IP detected inA549 cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10266-1-AP targets IFNGR2 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen IFNGR2 fusion protein Ag0317
Full Name interferon gamma receptor 2 (interferon gamma transducer 1)
Calculated Molecular Weight 45 kDa
Observed Molecular Weight 50-55 kDa
GenBank Accession NumberBC003624
Gene Symbol IFNGR2
Gene ID (NCBI) 3460
RRIDAB_2121709
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Interferon-gamam (IFN-γ) is a cytokine critical for innate and adaptive immunity against viral and intracellular bacterial infections and for tumor control. Cellular responses to IFN-γ are activated through its interaction with a heterodimeric receptor consisting of two subunits, IFNGR1 and IFNGR2. IFNGR1 is the ligand-binding subunit which binds IFN-γ with high affinity, whereas IFNGR2 serves as the accessory subunit which attaches to IFN-γ with a significantly lower affinity. Two subunits bind one IFN-γ dimer. Defects in IFNGR2 are a cause of mendelian susceptibility to mycobacterial disease (MSMD), also known as familial disseminated atypical mycobacterial infection. (PMID: 17981204; 946248; 10888113)

Protocols

Product Specific Protocols
WB protocol for IFNGR2 antibody 10266-1-APDownload protocol
IHC protocol for IFNGR2 antibody 10266-1-APDownload protocol
IP protocol for IFNGR2 antibody 10266-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Science

Aberrant type 1 immunity drives susceptibility to mucosal fungal infections.

Authors - Timothy J Break
mouseWB

Nat Commun

DENR controls JAK2 translation to induce PD-L1 expression for tumor immune evasion.

Authors - Baiwen Chen
humanWB

Cancer Commun (Lond)

MAGE-C3 promotes cancer metastasis by inducing epithelial-mesenchymal transition and immunosuppression in esophageal squamous cell carcinoma.

Authors - Qingnan Wu
humanWB

PLoS One

Impact of adipose tissue or umbilical cord derived mesenchymal stem cells on the immunogenicity of human cord blood derived endothelial progenitor cells.

Authors - Kefang Tan
humanWB

Biomol Biomed

Identification of RUNX1 and IFNGR2 as prognostic-related biomarkers correlated with immune infiltration and subtype differentiation of low-grade glioma

Authors -
humanIF,WB

Sci Adv

Plasma membrane nanodeformations promote actin polymerization through CIP4/CDC42 recruitment and regulate type II IFN signaling

Authors - Benjamin Ledoux