AP1B1 Polyklonaler Antikörper

AP1B1 Polyklonal Antikörper für IF, IP, WB,ELISA

Wirt / Isotyp

Kaninchen / IgG

Getestete Reaktivität

human

Anwendung

WB, IP, IF, ELISA

Konjugation

Unkonjugiert

Publikationen

4

Kat-Nr. : 16932-1-AP

Synonyme

ADTB1, AP 1 complex subunit beta 1, AP105A, AP1B1, BAM22, Beta 1 adaptin, Beta adaptin 1, CLAPB2

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Galerie der Validierungsdaten

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  WB analysis of HEK-293 using 16932-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 16932-1-AP (AP1B1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 16932-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 16932-1-AP (AP1B1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 using 16932-1-AP

  IP result of anti-AP1B1 (IP:16932-1-AP, 4ug; Detection:16932-1-AP 1:300) with HEK-293 cells lysate 2320 ug.

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  IF Staining of HepG2 using 16932-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using 16932-1-AP (AP1B1 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Geprüfte Anwendungen

• Erfolgreiche Detektion in WB: HEK-293-Zellen
• Erfolgreiche IP: HEK-293-Zellen
• Erfolgreiche Detektion in IF: HepG2-Zellen

Empfohlene Verdünnung

• Western Blot (WB): WB : 1:500-1:2400
• Immunpräzipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunfluoreszenz (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Veröffentlichte Anwendungen

• WB: See 4 publications below

Produktinformation

16932-1-AP bindet in WB, IP, IF, ELISA AP1B1 und zeigt Reaktivität mit human

• Getestete Reaktivität: human
• In Publikationen genannte Reaktivität: human
• Wirt / Isotyp: Kaninchen / IgG
• Klonalität: Polyklonal
• Typ: Antikörper
• Immunogen: AP1B1 fusion protein Ag10417
• Vollständiger Name: adaptor-related protein complex 1, beta 1 subunit
• Berechnetes Molekulargewicht: 919 aa, 101 kDa
• Beobachtetes Molekulargewicht: 101 kDa
• GenBank-Zugangsnummer: BC046242
• Gene symbol: AP1B1
• Gene ID (NCBI): 162
• Konjugation: Unkonjugiert
• Form: Liquid
• Reinigungsmethode: Antigen-Affinitätsreinigung
• Lagerungspuffer: PBS mit 0.02% Natriumazid und 50% Glycerin pH 7.3.
• Lagerungsbedingungen: Bei -20°C lagern. Nach dem Versand ein Jahr lang stabil Aliquotieren ist bei -20^oC Lagerung nicht notwendig. 20ul Größen enthalten 0,1% BSA.

Hintergrundinformationen

AP1B1 is a subunit of the adaptor complex AP-1 and is a member of the adaptin protein family. Adaptor protein (AP) complexes are cytosolic heterotetramers that mediate the sorting of membrane proteins in the secretory and endocytic pathways. AP-1 is found at the cytoplasmic face of coated vesicles located at the Golgi complex, where it mediates both the recruitment of clathrin to the membrane and the recognition of sorting signals within the cytosolic tails of transmembrane receptors.

Publikationen

Species	Application	Title
human	WB	Nat Genet; Bidirectional genome-wide CRISPR screens reveal host factors regulating SARS-CoV-2, MERS-CoV and seasonal HCoVs; Authors - Antoine Rebendenne
human	WB	Am J Hum Genet; Recessive Mutations in AP1B1 Cause Ichthyosis, Deafness, and Photophobia.; Authors - Lynn M Boyden
human	WB	J Cell Biol; Cargo-selective SNX-BAR proteins mediate retromer trimer independent retrograde transport.; Authors - Arunas Kvainickas
human	WB	Oncogene; Regulated secretion of mutant p53 negatively affects T lymphocytes in the tumor microenvironment; Authors - Xiang Dong

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