Gamma Adaptin Polyclonal antibody

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• Gamma Adaptin Polyclonal antibody (13258-1-AP)

• KD/KO Validated

Gamma Adaptin Polyclonal Antibody for WB, IHC, ELISA

Cat No. 13258-1-AP Copied!

Publications (7)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Synonyms

AP1G1, Adaptor protein complex AP-1 subunit gamma-1, Adaptor-related protein complex 1 subunit gamma-1, ADTG, AP-1 complex subunit gamma-1

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  WB analysis of HEK-293 using 13258-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 13258-1-AP (AP1G1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of HEK-293 using 13258-1-AP

  WB result of Gamma Adaptin antibody (13258-1-AP; 1:100000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Gamma Adaptin transfected HEK-293 cells.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of mouse skeletal muscle using 13258-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 13258-1-AP (AP1G1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human pancreas cancer using 13258-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 13258-1-AP (Gamma adaptin Antibody) at dilution of 1:50 (under 40x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human pancreas cancer using 13258-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 13258-1-AP (Gamma adaptin Antibody) at dilution of 1:50 (under 10x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: HEK-293 cells, mouse skeletal muscle tissue
• Positive IHC detected in: human pancreas cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

13258-1-AP targets Gamma Adaptin in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag3696

  Product name: Recombinant human Gamma adaptin protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 476-825 aa of BC036283

  Sequence: VQVAAWCIGEYGDLLVSGQCEEEEPIQVTEDEVLDILESVLISNMSTSVTRGYALTAIMKLSTRFTCTVNRIKKVVSIYGSSIDVELQQRAVEYNALFKKYDHMRSALLERMPVMEKVTTNGPTEIVQTNGETEPAPLETKPPPSGPQPTSQANDLLDLLGGNDITPVIPTAPTSKPSSAGGELLDLLGDINLTGAPAAAPAPASVPQISQPPFLLDGLSSQPLFNDIAAGIPSITAYSKNGLKIEFTFERSNTNPSVTVITIQASNSTELDMTDFVFQAAVPKTFQLQLLSPSSSIVPAFNTGTITQVIKVLNPQKQQLRMRIKLTYNHKGSAMQDLAEVNNFPPQSWQ

• Full Name: adaptor-related protein complex 1, gamma 1 subunit
• Calculated Molecular Weight: 825 aa, 92 kDa
• Observed Molecular Weight: 91 kDa
• GenBank Accession Number: BC036283
• Gene Symbol: Gamma Adaptin
• Gene ID (NCBI): 164
• RRID: AB_2058209
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O43747
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

What published studies show

The Gamma Adaptin Polyclonal antibody (Cat# 13258-1-AP) has 7 citations published in Nature Cell Biology, PNAS, Cell Reports, Cells, Frontiers in Cell and Developmental Biology, Frontiers in Cellular Neuroscience, and bioRxiv. Research fields include endosomal recycling, vesicular trafficking, Golgi retention and oncogenic signaling in GIST cells, T cell receptor distribution, trans-Golgi network protein sorting, and cerebellar development. Applications cited are primarily WB and IF.

Automatically generated from the 7 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Nat Cell Biol; Molecular identification of a BAR domain-containing coat complex for endosomal recycling of transmembrane proteins.; Authors - Boris Simonetti
human	IF	Proc Natl Acad Sci U S A; An in vitro vesicle formation assay reveals cargo clients and factors that mediate vesicular trafficking.; Authors - Yan Huang
human	WB	Cell Rep; Golgi retention and oncogenic KIT signaling via PLCγ2-PKD2-PI4KIIIβ activation in gastrointestinal stromal tumor cells; Authors - Yuuki Obata
human	WB	Cells; Five Inhibitory Receptors Display Distinct Vesicular Distributions in Murine T Cells; Authors - Jiahe Lu
human	IF	Front Cell Dev Biol; Visualization of Protein Sorting at the Trans-Golgi Network and Endosomes Through Super-Resolution Imaging.; Authors - Yan Huang
mouse	WB	Front Cell Neurosci; MEA6 Deficiency Impairs Cerebellar Development and Motor Performance by Tethering Protein Trafficking.; Authors - Xin-Tai Wang