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  • Featured Product
  • KD/KO Validated

NF-κB p65 Polyclonal antibody

NF-κB p65 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (7)

Applications

WB, IHC, IF/ICC, IP, CoIP, ChIP, ELISA

Conjugate

Unconjugated

Cat no : 10745-1-AP

Synonyms

NF κB, p65, p65; RELA, RELA, NF Kappa B

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of Hek-293 cells using NF-κB p65 Polyclonal antibody (10745-1-AP)

    WB analysis of Hek-293 cells using 10745-1-AP

    WB result of RELA, p65antibody (10745-1-AP, 1:500) with si-Control and si-RELA,p65 transfected HeLa cells.

  • Western Blot (WB) analysis of various lysates using NF-κB p65 Polyclonal antibody (10745-1-AP)

    WB analysis using 10745-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 10745-1-AP (NF-κB p65 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of MCF-7 cells using NF-κB p65 Polyclonal antibody (10745-1-AP)

    WB analysis of MCF-7 using 10745-1-AP

    MCF7 cells were subjected to SDS PAGE followed by western blot with 10745-1-AP (p65 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of K-562 cells using NF-κB p65 Polyclonal antibody (10745-1-AP)

    WB analysis of K-562 using 10745-1-AP

    K-562 cells were subjected to SDS PAGE followed by western blot with 10745-1-AP (p65 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Jurkat cells using NF-κB p65 Polyclonal antibody (10745-1-AP)

    WB analysis of Jurkat using 10745-1-AP

    Jurkat cells were subjected to SDS PAGE followed by western blot with 10745-1-AP (p65 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HeLa cells using NF-κB p65 Polyclonal antibody (10745-1-AP)

    IP experiment of HeLa using 10745-1-AP

    IP result of anti-NF-κB p65 (IP:10745-1-AP, 3ug; Detection:10745-1-AP 1:1000) with HeLa cells lysate 5000ug.

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using NF-κB p65 Polyclonal antibody (10745-1-AP)

    IHC staining of human breast cancer using 10745-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 10745-1-AP (NF-κB p65 antibody) at dilution of 1:200 (under 0x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human colon tissue using NF-κB p65 Polyclonal antibody (10745-1-AP)

    IHC staining of human colon using 10745-1-AP

    Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 10745-1-AP (NF-κB p65 antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Sodium Citrate buffer (pH 6.0).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using NF-κB p65 Polyclonal antibody (10745-1-AP)

    IHC staining of human breast cancer using 10745-1-AP

    Immunohistochemical analysis of paraffin-embedded human breast cancer using 10745-1-AP (p65 antibody) at dilution of 1:100 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human stomach tissue using NF-κB p65 Polyclonal antibody (10745-1-AP)

    IHC staining of human stomach using 10745-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach using 10745-1-AP (p65 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human stomach tissue using NF-κB p65 Polyclonal antibody (10745-1-AP)

    IHC staining of human stomach using 10745-1-AP

    Immunohistochemical analysis of paraffin-embedded human stomach using 10745-1-AP (p65 antibody) at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using NF-κB p65 Polyclonal antibody (10745-1-AP)

    IHC staining of human liver cancer using 10745-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer using 10745-1-AP (p65 antibody) at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human liver cancer tissue using NF-κB p65 Polyclonal antibody (10745-1-AP)

    IHC staining of human liver cancer using 10745-1-AP

    Immunohistochemical analysis of paraffin-embedded human liver cancer using 10745-1-AP (p65 antibody) at dilution of 1:50 (under 40x lens).

  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using NF-κB p65 Polyclonal antibody (10745-1-AP)

    IF Staining of HepG2 using 10745-1-AP

    Immunofluorescent analysis of (-20℃ Ethanol) fixed HepG2 cells using 10745-1-AP (p65; RELA antibody) at dilution of 1:100 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of HT-1080 cells using NF-κB p65 Polyclonal antibody (10745-1-AP)

    IF Staining of HT-1080 using 10745-1-AP

    Immunofluorescent analysis of (4% PFA) fixed TNF alpha treated HT-1080 cells using NF-κB p65 antibody (10745-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red).

Tested Applications

Positive WB detected inA431 cells, Jurkat cells, MCF-7 cells, K-562 cells, Hek-293 cells, HeLa cells, Raji cells, NIH/3T3 cells
Positive IP detected inHeLa cells
Positive IHC detected inhuman breast cancer tissue, human colon tissue, human liver cancer tissue, human stomach tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inHepG2 cells, TNF alpha treated HT-1080 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10745-1-AP targets NF-κB p65 in WB, IHC, IF/ICC, IP, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, rabbit, monkey, chicken, hamster, sheep, goat, fish
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen NF-κB p65 fusion protein Ag1199
Full Name v-rel reticuloendotheliosis viral oncogene homolog A (avian)
Calculated Molecular Weight 65 kDa
Observed Molecular Weight 65 kDa
GenBank Accession NumberBC011603
Gene Symbol NF-κB p65
Gene ID (NCBI) 5970
RRIDAB_2178878
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Nuclear factor k B (NF-kB) is a sequence-specific DNA-binding protein complex which regulates the expression of viral genomes, including the human immunodeficiency virus, and a variety of cellular genes, particularly those involved in immune and inflammatory responses. The members of the NF-kB family in mammalian cells include the proto-oncogene c-Rel,p50/p105 (NFkB1), p65 (RelA), p52/p100 (NFkB2), and RelB. All of these proteins share a conserved 300-amino acid region known as the Rel homology domain which is responsible for DNA binding, dimerization, and nuclear translocation of NF-kB. The p65 subunit is a major component of NF-kB complexes and is responsible for trans-activation. NF-kB heterodimeric p65-p50 and p65-c-Rel complexes are transcriptional activators. The NF-kB p65-p65 complex appears to be involved in invasin-mediated activation of IL-8 expression. The inhibitory effect of IkB upon NF-kB the cytoplasm is exerted primarily through the interaction with p65. p65 shows a weak DNA-binding site which could contribute directly to DNA binding in the NF-kB complex. It associates with chromatin at the NF-kB promoter region via association with DDX1. This antibody is a rabbit polyclonal antibody raised against residues near the N terminus of human RELA.

Protocols

Product Specific Protocols
WB protocol for NF-κB p65 antibody 10745-1-APDownload protocol
IHC protocol for NF-κB p65 antibody 10745-1-APDownload protocol
IF protocol for NF-κB p65 antibody 10745-1-APDownload protocol
IP protocol for NF-κB p65 antibody 10745-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Bioact Mater

Silicate ions as soluble form of bioactive ceramics alleviate aortic aneurysm and dissection

Authors - Yumei Que
View Article
mouseIF

Blood

PMID: 32291445

Authors - Kunpeng Wu
View Article
mouseWB

Mol Cell

Serine synthesis sustains macrophage IL-1β production via NAD+-dependent protein acetylation

Authors - Chuanlong Wang
View Article
humanWB

Nat Microbiol

IFI16 directly senses viral RNA and enhances RIG-I transcription and activation to restrict influenza virus infection.

Authors - Zhimin Jiang
View Article
humanWB

Adv Sci (Weinh)

OR11H1 Missense Variant Confers the Susceptibility to Vogt-Koyanagi-Harada Disease by Mediating Gadd45g Expression

Authors - Xingran Li
View Article
humanWB

Sci Adv

JLP-centrosome is essential for the microtubule-mediated nucleocytoplasmic transport induced by extracellular stimuli.

Authors - Clement M Lee
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

FH

Maximilian (Verified Customer) (06-30-2023)

Quite a bit of unspecific binding. Did not work in mouse cell lines.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Mainly Human Breast Cancer Cell lines (MDA-MB-436, MDA-MB-468, MDA-MB-231)
FH

Xie (Verified Customer) (09-29-2022)

NF-κB p65 Antibody staining.

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:250
  • Cell Tissue Type: hela
NF-κB p65 Antibody Immunofluorescence validation (1:250 dilution) in hela (Cat no:10745-1-AP)
FH

Murali (Verified Customer) (01-24-2022)

Very nice antibody for IHC

  • Applications: Immunohistochemistry
  • Primary Antibody Dilution: 1 to 200
  • Cell Tissue Type: pharyngeal mucosal
NF-κB p65 Antibody Immunohistochemistry validation (1 to 200 dilution) in pharyngeal mucosal (Cat no:10745-1-AP)
FH

Azita (Verified Customer) (05-31-2021)

NSC-34 cells (motor neuron-like cells) strong labelling in WB

  • Applications: Western Blot
  • Primary Antibody Dilution: 1/2000
  • Cell Tissue Type: Human spinal cord (cervical) tissue
FH

Zehua (Verified Customer) (12-17-2019)

After siRNA treatment for 48 hours, specific signal shown here. Works excellent!

  • Applications: Western Blot, Cell culture,
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: K562
NF-κB p65 Antibody Western Blot,Cell culture, validation (1:1000 dilution) in K562 (Cat no:10745-1-AP)
FH

Feng-qian (Verified Customer) (12-07-2018)

  • Applications: Western Blot, Immunofluorescence,
  • Primary Antibody Dilution: 1/500
  • Cell Tissue Type: HaCaT
FH

Fraser (Verified Customer) (06-06-2018)

  • Applications: Other (please state below),