CoraLite®594-conjugated NF-κB p65 Polyclonal antibody

• Featured Product
• KD/KO Validated

NF-κB p65 Polyclonal Antibody for IF/ICC

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, pig

Applications

IF/ICC

Conjugate

CoraLite®594 Fluorescent Dye

Publications

2

Cat no : CL594-10745

Synonyms

NF κB, p65, p65; RELA, RELA, NF Kappa B

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Validation Data Gallery

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  IF Staining of HepG2 using CL594-10745

  Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using CoraLite®594 NF-κB p65 antibody (CL594-10745) at dilution of 1:100, CoraLite®488 Beta Actin antibody (CL488-66009, Clone: 2D4H5, green). DAPI (Blue).

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  IF Staining of HT-1080 using CL594-10745

  Immunofluorescent analysis of (4% PFA) fixed TNF alpha treated HT-1080 cells using CoraLite®594 NF-κB p65 antibody (CL594-10745) at dilution of 1:200.

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  IF Staining of HepG2 using CL594-10745

  Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using CoraLite®594 NF-κB p65 antibody (CL594-10745) at dilution of 1:200.

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Tested Applications

• Positive IF/ICC detected in: HepG2 cells, TNF alpha treated HT-1080 cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IF: See 2 publications below

Product Information

CL594-10745 targets NF-κB p65 in IF/ICC applications and shows reactivity with human, mouse, rat, pig samples.

• Tested Reactivity: human, mouse, rat, pig
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: NF-κB p65 fusion protein Ag1199
• Full Name: v-rel reticuloendotheliosis viral oncogene homolog A (avian)
• Calculated Molecular Weight: 65 kDa
• Observed Molecular Weight: 65 kDa
• GenBank Accession Number: BC011603
• Gene Symbol: NF-κB p65
• Gene ID (NCBI): 5970
• RRID: AB_2919744
• Conjugate: CoraLite®594 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 588 nm / 604 nm
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20^oC storage.

Background Information

Nuclear factor k B (NF-kB) is a sequence-specific DNA-binding protein complex which regulates the expression of viral genomes, including the human immunodeficiency virus, and a variety of cellular genes, particularly those involved in immune and inflammatory responses. The members of the NF-kB family in mammalian cells include the proto-oncogene c-Rel,p50/p105 (NFkB1), p65 (RelA), p52/p100 (NFkB2), and RelB. All of these proteins share a conserved 300-amino acid region known as the Rel homology domain which is responsible for DNA binding, dimerization, and nuclear translocation of NF-kB. The p65 subunit is a major component of NF-kB complexes and is responsible for trans-activation. NF-kB heterodimeric p65-p50 and p65-c-Rel complexes are transcriptional activators. The NF-kB p65-p65 complex appears to be involved in invasin-mediated activation of IL-8 expression. The inhibitory effect of IkB upon NF-kB the cytoplasm is exerted primarily through the interaction with p65. p65 shows a weak DNA-binding site which could contribute directly to DNA binding in the NF-kB complex. It associates with chromatin at the NF-kB promoter region via association with DDX1. This antibody is a rabbit polyclonal antibody raised against residues near the N terminus of human RELA.

Publications

Species	Application	Title
mouse	IF	Front Med (Lausanne); New, potent, small molecule agonists of tyrosine kinase receptors attenuate dry eye disease; Authors - Zhiyuan Yu
mouse	IF	Invest Ophthalmol Vis Sci; Induction of Innate Inflammatory Pathways in the Corneal Epithelium in the Desiccating Stress Dry Eye Model; Authors - Zhiyuan Yu