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CoraLite® Plus 488-conjugated TUBB3-specific/TUJ1 Monoclonal antibody

TUBB3-specific/TUJ1 Monoclonal Antibody for WB, IF-P

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse, rat

Applications

WB, IF-P

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

1F8G10

Cat no : CL488-66375

Synonyms

beta 4, beta tubulin, MC1R, TUBB, TUBB3, TUBB3-specific, TUBB4, Tubulin beta 3 chain, Tubulin beta 4 chain, Tubulin beta III, tubulin, beta 3, tuj1, tuj1 antibody

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using CoraLite® Plus 488-conjugated TUBB3-specific/TUJ1 (CL488-66375)

    WB analysis using CL488-66375

    Neuro-2a cell lysates were subjected to SDS PAGE followed by western blot with CL488-66375 (TUBB3-specific antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using CoraLite® Plus 488-conjugated TUBB3-specific/TUJ1 (CL488-66375)

    IF Staining of mouse brain using CL488-66375

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CL488-66375 (TUBB3-specific antibody) at dilution of 1:50.

  • Immunofluorescence (IF) / fluorescent staining of mouse brain tissue using CoraLite® Plus 488-conjugated TUBB3-specific/TUJ1 (CL488-66375)

    IF Staining of mouse brain using CL488-66375

    Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CL488-66375 (TUBB3-specific antibody) at dilution of 1:50.

  • Immunofluorescence (IF) / fluorescent staining of mouse spine using CoraLite® Plus 488-conjugated TUBB3-specific/TUJ1 (CL488-66375)

    IF Staining of mouse spine using CL488-66375

    E14.5 FFPE mouse spine stained for beta-III tubulin (red, Cat. No CL488-66375) and GFAP (cyan, Cat. No 60190-1-Ig). Beta-III tubulin stains neurons and was conjugated to CoraLite-488 fluorescent dye and pseudocolored to red. GFAP stains astrocytes along the spinal column. In this image, astrocyte projections can be seen among spinal neurons in the spinal cord. Image credit: @Immunofluorescence on Instagram.

  • Immunofluorescence (IF) / fluorescent staining of mouse cerebellum tissue using CoraLite® Plus 488-conjugated TUBB3-specific/TUJ1 (CL488-66375)

    IF Staining of mouse cerebellum using CL488-66375

    Neonatal mouse cerebellum stained for beta-III tubulin (magenta, Cat. No CL488-66375) and GFAP (green, Cat. No 60190-1-Ig). Beta-III tubulin stains neurons and was conjugated to CoraLite-488 fluorescent dye and pseudocolored to magenta. GFAP stains astrocytes. In this image, astrocyte projections can be seen intermingling with neurons. Image credit: @Immunofluorescence on Instagram.

  • Immunofluorescence (IF) / fluorescent staining of mouse testis tissue using CoraLite® Plus 488-conjugated TUBB3-specific/TUJ1 (CL488-66375)

    IF Staining of mouse testis using CL488-66375

    Adult mouse testes were co-stained with TUBB3 (CL488-66375, in green), DDX4/VASA (51042-1-AP, in magenta), and PCNA (60097-1-Ig, in blue). TUBB3/Beta III tubulin marks the Sertoli cells (structural support cells), VASA marks the germ cells (developing sperm), and PCNA marks proliferating cells (these cells are at the base of the tubule, where the sperm stem cells are located). The image was created in paid partnership with @Immunofluorescence on Instagram.

Tested Applications

Positive WB detected inNeuro-2a cells
Positive IF-P detected inmouse brain tissue, mouse spine, mouse cerebellum tissue, mouse testis tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunofluorescence (IF)-PIF-P : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-66375 targets TUBB3-specific/TUJ1 in WB, IF-P applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG1
Class Monoclonal
Type Antibody
Immunogen Peptide
Full Name tubulin, beta 3
Calculated Molecular Weight 55 kDa
GenBank Accession NumberNM_001197181
Gene Symbol TUBB3
Gene ID (NCBI) 10381
RRIDAB_2883321
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein G purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

TUBB3, the class III β tubulin or Tuj1, is selectively expressed in testis and neurons of the central and peripheral nervous system. It has been widely used as a marker for neurons. Aberrant expression of TUBB3 has also been found in various tumors of non-neural origin and can be used as a biomarker for cancer aggressiveness and a marker for the tendency to respond poorly to chemotherapy. This antibody is specific to TUBB3 but not cross-react with other tubulin isoforms. And the antibody is conjugated with CL488, Ex/Em 488 nm/515 nm.

Protocols

Product Specific Protocols
WB protocol for CL Plus 488 TUBB3-specific/TUJ1 antibody CL488-66375Download protocol
IF protocol for CL Plus 488 TUBB3-specific/TUJ1 antibody CL488-66375Download protocol
Standard Protocols
Click here to view our Standard Protocols