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  • Featured Product
  • KD/KO Validated

p63 Polyclonal antibody

p63 Polyclonal Antibody for WB, IF/ICC, IF-P, FC (Intra), IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, IF-P, FC (Intra), IP, CoIP, ChIP, ELISA

Conjugate

Unconjugated

Cat no : 12143-1-AP

Synonyms

TP63, tumor protein p63, Tumor protein 63, Transformation-related protein 63, TP 63

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using p63 Polyclonal antibody (12143-1-AP)

    WB analysis using 12143-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12143-1-AP (p63 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using p63 Polyclonal antibody (12143-1-AP)

    WB analysis using 12143-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12143-1-AP (p63 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using p63 Polyclonal antibody (12143-1-AP)

    WB analysis using 12143-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 12143-1-AP (p63 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of Apoptosised HeLa cells using p63 Polyclonal antibody (12143-1-AP)

    WB analysis of Apoptosised HeLa using 12143-1-AP

    Apoptosised HeLa cells were subjected to SDS PAGE followed by western blot with 12143-1-AP (p63 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse heart tissue using p63 Polyclonal antibody (12143-1-AP)

    WB analysis of mouse heart using 12143-1-AP

    mouse heart tissue were subjected to SDS PAGE followed by western blot with 12143-1-AP (TP63 Antibody) at dilution of 1:1000 incubated at 4 degree celsius over night.

  • Western Blot (WB) analysis of human heart tissue using p63 Polyclonal antibody (12143-1-AP)

    WB analysis of human heart using 12143-1-AP

    human heart tissue were subjected to SDS PAGE followed by western blot with 12143-1-AP (p63 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of U-937 cells using p63 Polyclonal antibody (12143-1-AP)

    WB analysis of U-937 using 12143-1-AP

    U-937 cells were subjected to SDS PAGE followed by western blot with 12143-1-AP (p63 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of A431 cells using p63 Polyclonal antibody (12143-1-AP)

    IP experiment of A431 using 12143-1-AP

    IP result of anti-p63 (IP:12143-1-AP, 4ug; Detection:12143-1-AP 1:1000) with A431 cells lysate 1200 ug.

  • Immunoprecipitation (IP) experiment of A431 cells using p63 Polyclonal antibody (12143-1-AP)

    IP experiment of A431 using 12143-1-AP

    IP result of anti-p63 (IP:12143-1-AP, 4ug; Detection:12143-1-AP 1:300) with A431 cells lysate 1200ug.

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using p63 Polyclonal antibody (12143-1-AP)

    IHC staining of human tonsillitis using 12143-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 12143-1-AP (p63 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human tonsillitis tissue using p63 Polyclonal antibody (12143-1-AP)

    IHC staining of human tonsillitis using 12143-1-AP

    Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 12143-1-AP (p63 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using p63 Polyclonal antibody (12143-1-AP)

    IHC staining of human prostate cancer using 12143-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 12143-1-AP (p63 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using p63 Polyclonal antibody (12143-1-AP)

    IHC staining of human prostate cancer using 12143-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 12143-1-AP (p63 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human bowen disease using p63 Polyclonal antibody (12143-1-AP)

    IHC staining of human bowen disease using 12143-1-AP

    Immunohistochemical analysis of paraffin-embedded human bowen disease slide using 12143-1-AP (p63 antibody) at dilution of 1:300 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human bowen disease using p63 Polyclonal antibody (12143-1-AP)

    IHC staining of human bowen disease using 12143-1-AP

    Immunohistochemical analysis of paraffin-embedded human bowen disease slide using 12143-1-AP (p63 antibody) at dilution of 1:300 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunofluorescence (IF) / fluorescent staining of human lung cancer tissue using p63 Polyclonal antibody (12143-1-AP)

    IF Staining of human lung cancer using 12143-1-AP

    Immunofluorescent analysis of (4% PFA) fixed human lung cancer tissue using p63 antibody (12143-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of human lung cancer tissue using p63 Polyclonal antibody (12143-1-AP)

    IF Staining of human lung cancer using 12143-1-AP

    Immunofluorescent analysis of (4% PFA) fixed human lung cancer tissue using p63 antibody (12143-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of A431 cells using p63 Polyclonal antibody (12143-1-AP)

    IF Staining of A431 using 12143-1-AP

    Immunofluorescent analysis of (4% PFA) fixed A431 cells using p63 antibody (12143-1-AP) at dilution of 1:1000 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-Phalloidin (red).

  • Flow cytometry (FC) experiment of A431 cells using p63 Polyclonal antibody (12143-1-AP)

    FC experiment of A431 using 12143-1-AP

    1X10^6 A431 cells were intracellularly stained with 0.4 ug Anti-Human p63 (12143-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

Tested Applications

Positive WB detected inA431 cells, human heart tissue, mouse heart tissue, U-937 cells, Apoptosised HeLa cells, HaCaT cells, PC-12 cells, mouse thymus tissue
Positive IP detected inA431 cells
Positive IHC detected inhuman tonsillitis tissue, human prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inhuman lung cancer tissue
Positive IF/ICC detected inA431 cells
Positive FC (Intra) detected inA431 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)-PIF-P : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:500-1:2000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

12143-1-AP targets p63 in WB, IF/ICC, IF-P, FC (Intra), IP, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen p63 fusion protein Ag2791
Full Name tumor protein p63
Calculated Molecular Weight 680 aa, 77 kDa
Observed Molecular Weight 63-68 kDa
GenBank Accession NumberBC039815
Gene Symbol TP63
Gene ID (NCBI) 8626
RRIDAB_10597397
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TP63, also named KET, P63, P73H, P73L and TP73L, belongs to the p53 family. It is a homologue of the tumor suppressor p53 and p73 genes. It is involved in malignancy acquisition and maintenance of cells. Unlike p53, the p63 gene encodes multiple isotypes with remarkably divergent abilities to transactivate p53 reporter genes and induce apoptosis. TP63 acts as a sequence specific DNA binding transcriptional activator or repressor. TP63 has 12 isoforms with MW 40kd(P40), 50kd(P60),63kd(P63) and 73kd(P73L). It is Nuclear stain.

Protocols

Product Specific Protocols
WB protocol for p63 antibody 12143-1-APDownload protocol
IHC protocol for p63 antibody 12143-1-APDownload protocol
IF protocol for p63 antibody 12143-1-APDownload protocol
IP protocol for p63 antibody 12143-1-APDownload protocol
FC protocol for p63 antibody 12143-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouse

Nature

Divergent transcriptional regulation of astrocyte reactivity across disorders.

Authors - Joshua E Burda
View Article
humanWB,IP,ChIP

Nucleic Acids Res

Activation of bivalent factor DLX5 cooperates with master regulator TP63 to promote squamous cell carcinoma.

Authors - Yongsheng Huang
  • KD Validated
View Article
humanIF

Cell Rep

A Wnt-induced lncRNA-DGCR5 splicing switch drives tumor-promoting inflammation in esophageal squamous cell carcinoma

Authors - Yue Li
View Article
mouse

Oncogene

TP53, CDKN2A/P16, and NFE2L2/NRF2 regulate the incidence of pure- and combined-small cell lung cancer in mice.

Authors - Samera H Hamad
View Article
humanIF

Elife

Chloride channels regulate differentiation and barrier functions of the mammalian airway.

Authors - Mu He
View Article
human

Oncogene

Circulating IGF-1 promotes prostate adenocarcinoma via FOXO3A/BIM signaling in a double-transgenic mouse model.

Authors - Shuang Wang
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

FH

Tanusree (Verified Customer) (12-18-2019)

Product worked well in WB at 1:500 dilution

  • Applications: Western Blot,
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: Mouse brain
FH

Kui (Verified Customer) (09-19-2019)

Best P63 Ab I can find so far.

  • Applications: Immunofluorescence,
  • Primary Antibody Dilution: 1:200
  • Cell Tissue Type: Mouse Esophagus
p63 Antibody Immunofluorescence, validation (1:200 dilution) in Mouse Esophagus (Cat no:12143-1-AP)