TH Polyclonal antibody
TH Polyclonal Antibody for WB, IHC, IF-P, IP, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat and More (3)
Applications
WB, IHC, IF-P, IP, ELISA
Conjugate
Unconjugated
145
Cat no : 25859-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | mouse brain tissue, rat brain tissue |
Positive IP detected in | mouse brain tissue |
Positive IHC detected in | mouse brain tissue, mouse heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF-P detected in | mouse brain tissue |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:2000-1:10000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:5000-1:20000 |
Immunofluorescence (IF)-P | IF-P : 1:200-1:800 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
WB | See 89 publications below |
IHC | See 50 publications below |
IF | See 54 publications below |
ELISA | See 1 publications below |
Product Information
25859-1-AP targets TH in WB, IHC, IF-P, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, rat, canine, zebrafish, gerbil |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | TH fusion protein Ag23075 |
Full Name | tyrosine hydroxylase |
Calculated Molecular Weight | 528 aa, 59 kDa |
Observed Molecular Weight | 50-55 kDa |
GenBank Accession Number | BC104967 |
Gene Symbol | TH |
Gene ID (NCBI) | 7054 |
RRID | AB_2716568 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
TH(Tyrosine 3-monooxygenase) converts L-tyrosine to L-3,4-dihydroxyphenylalanine (L-DOPA), the essential and rate-limiting step to formation of dopamine and other catecholamines. TH plays an important role in the physiology of adrenergic neurons and can be used as a marker for dopaminergic and noradrenergic neurons. This protein has 6 isoforms produced by alternative splicing with the MW from 44 kDa to 58 kDa.
Protocols
Product Specific Protocols | |
---|---|
WB protocol for TH antibody 25859-1-AP | Download protocol |
IHC protocol for TH antibody 25859-1-AP | Download protocol |
IF protocol for TH antibody 25859-1-AP | Download protocol |
IP protocol for TH antibody 25859-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Nat Commun Imprinted lncRNA Dio3os preprograms intergenerational brown fat development and obesity resistance. | ||
Nat Commun NAc-VTA circuit underlies emotional stress-induced anxiety-like behavior in the three-chamber vicarious social defeat stress mouse model. | ||
Nat Commun Locus coeruleus-CA1 projections are involved in chronic depressive stress-induced hippocampal vulnerability to transient global ischaemia. | ||
Brain Reactive microglia enhance the transmission of exosomal alpha-synuclein via toll-like receptor 2. | ||
Acta Biomater Biomaterial based strategies to reconstruct the nigrostriatal pathway in organotypic slice co-cultures. | ||
Redox Biol Targeting mitochondria-inflammation circle by renal denervation reduces atheroprone endothelial phenotypes and atherosclerosis. |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Kenzo (Verified Customer) (01-05-2024) | This antibody worked well for immunolabeling on mouse brain tissue sections
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FH Raphael (Verified Customer) (08-02-2021) | This antibody is good for western blot. The dilution at 1/1000 was too strong but at 1/2000 it works perfectly.
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FH Teresa (Verified Customer) (11-03-2017) | Standard protocol, blocked with 1% goat serum in TBS, heat-mediated antigen retrieval
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FH Teresa (Verified Customer) (10-16-2017) | Signal was strong and specific. Could probably dilute further.
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