TH Polyclonal antibody

TH Polyclonal Antibody for WB, IHC, IF-P, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (3)

Applications

WB, IHC, IF-P, IP, ELISA

Conjugate

Unconjugated

Cat no : 25859-1-AP

Synonyms

tyrosine hydroxylase, Tyrosine 3 monooxygenase, Tyrosine 3 hydroxylase, TYH, EC:1.14.16.2



Tested Applications

Positive WB detected inmouse brain tissue, rat brain tissue
Positive IP detected inmouse brain tissue
Positive IHC detected inmouse brain tissue, mouse heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF-P detected inmouse brain tissue

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:5000-1:20000
Immunofluorescence (IF)-PIF-P : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

25859-1-AP targets TH in WB, IHC, IF-P, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, canine, zebrafish, gerbil
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen TH fusion protein Ag23075
Full Name tyrosine hydroxylase
Calculated Molecular Weight 528 aa, 59 kDa
Observed Molecular Weight 50-55 kDa
GenBank Accession NumberBC104967
Gene Symbol TH
Gene ID (NCBI) 7054
RRIDAB_2716568
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TH(Tyrosine 3-monooxygenase) converts L-tyrosine to L-3,4-dihydroxyphenylalanine (L-DOPA), the essential and rate-limiting step to formation of dopamine and other catecholamines. TH plays an important role in the physiology of adrenergic neurons and can be used as a marker for dopaminergic and noradrenergic neurons. This protein has 6 isoforms produced by alternative splicing with the MW from 44 kDa to 58 kDa.

Protocols

Product Specific Protocols
WB protocol for TH antibody 25859-1-APDownload protocol
IHC protocol for TH antibody 25859-1-APDownload protocol
IF protocol for TH antibody 25859-1-APDownload protocol
IP protocol for TH antibody 25859-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Nat Commun

Imprinted lncRNA Dio3os preprograms intergenerational brown fat development and obesity resistance.

Authors - Yan-Ting Chen
mouseIF

Nat Commun

NAc-VTA circuit underlies emotional stress-induced anxiety-like behavior in the three-chamber vicarious social defeat stress mouse model.

Authors - Guangjian Qi
mouseIF

Nat Commun

Locus coeruleus-CA1 projections are involved in chronic depressive stress-induced hippocampal vulnerability to transient global ischaemia.

Authors - Qian Zhang
mouseWB

Brain

Reactive microglia enhance the transmission of exosomal alpha-synuclein via toll-like receptor 2.

Authors - Yun Xia
mouseIHC

Acta Biomater

Biomaterial based strategies to reconstruct the nigrostriatal pathway in organotypic slice co-cultures.

Authors - Buket Ucar
mouseIHC

Redox Biol

Targeting mitochondria-inflammation circle by renal denervation reduces atheroprone endothelial phenotypes and atherosclerosis.

Authors - Zhuqing Li

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

Kenzo (Verified Customer) (01-05-2024)

This antibody worked well for immunolabeling on mouse brain tissue sections

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:5000
  • Cell Tissue Type: Mouse brain
FH

Raphael (Verified Customer) (08-02-2021)

This antibody is good for western blot. The dilution at 1/1000 was too strong but at 1/2000 it works perfectly.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1/2000
  • Cell Tissue Type: Rat brain
TH Antibody Western Blot validation (1/2000 dilution) in Rat brain (Cat no:25859-1-AP)
FH

Teresa (Verified Customer) (11-03-2017)

Standard protocol, blocked with 1% goat serum in TBS, heat-mediated antigen retrieval

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:200
  • Cell Tissue Type: wildtype mouse brain
TH Antibody Immunofluorescence validation (1:200 dilution) in wildtype mouse brain (Cat no:25859-1-AP)
FH

Teresa (Verified Customer) (10-16-2017)

Signal was strong and specific. Could probably dilute further.

  • Applications: Immunohistochemistry
  • Primary Antibody Dilution: 1:200
  • Cell Tissue Type: mouse brain
TH Antibody Immunohistochemistry validation (1:200 dilution) in mouse brain (Cat no:25859-1-AP)