DOPA decarboxylase Polyclonal antibody

DOPA decarboxylase Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugate

Unconjugated

Publications

10

Cat no : 10166-1-AP

Synonyms

DDC, EC:4.1.1.28, Aromatic-L-amino-acid decarboxylase, AADC

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Validation Data Gallery

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  WB analysis of mouse brain using 10166-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 10166-1-AP (DOPA decarboxylase antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 10166-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10166-1-AP (DOPA decarboxylase antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 10166-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10166-1-AP (DOPA decarboxylase antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 10166-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 10166-1-AP (DOPA decarboxylase antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 10166-1-AP

  IP result of anti-DOPA decarboxylase (IP:10166-1-AP, 4ug; Detection:10166-1-AP 1:800) with mouse brain tissue lysate 4000ug.

• 

  IHC staining of rat small intestine using 10166-1-AP

  Immunohistochemical analysis of paraffin-embedded rat small intestine tissue slide using 10166-1-AP (DOPA decarboxylase antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 10166-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 10166-1-AP (DOPA decarboxylase antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse kidney using 10166-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 10166-1-AP (DOPA decarboxylase antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat small intestine using 10166-1-AP

  Immunohistochemical analysis of paraffin-embedded rat small intestine tissue slide using 10166-1-AP (DOPA decarboxylase antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat kidney using 10166-1-AP

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 10166-1-AP (DOPA decarboxylase antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat kidney using 10166-1-AP

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 10166-1-AP (DOPA decarboxylase antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 10166-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10166-1-AP (DOPA decarboxylase antibody at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver cancer using 10166-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 10166-1-AP (DOPA decarboxylase antibody at dilution of 1:50 (under 40x lens).

• 

  IF Staining of SH-SY5Y using 10166-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using DOPA decarboxylase antibody (10166-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: SH-SY5Y cells, mouse kidney tissue, mouse brain tissue, rat kidney tissue, PC-12 cells
• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: mouse kidney tissue, human liver cancer tissue, rat kidney tissue, rat small intestine tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: SH-SY5Y cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 5 publications below
• IHC: See 7 publications below
• IF: See 2 publications below

Product Information

10166-1-AP targets DOPA decarboxylase in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: DOPA decarboxylase fusion protein Ag0219
• Full Name: dopa decarboxylase (aromatic L-amino acid decarboxylase)
• Calculated Molecular Weight: 54 kDa
• Observed Molecular Weight: 48-50 kDa
• GenBank Accession Number: BC008366
• Gene Symbol: DOPA decarboxylase
• Gene ID (NCBI): 1644
• RRID: AB_2088937
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Aromatic-L-amino-acid decarboxylase belongs to the pyridoxal-dependent aminotransferase superfamily.DDC catalyzes the decarboxylation of L-3,4-dihydroxyphenylalanine (DOPA) to dopamine, L-5-hydroxytryptophan to serotonin and L-tryptophan to tryptamine.DDC is the cause of aromatic L-amino-acid decarboxylase deficiency (AADCD).Researches showed that Ddc is only one of the enzymes in the biosynthetic pathways for bioamines and catecholamines.

Publications

Species	Application	Title
mouse	IF	Nature; Reversing a model of Parkinson's disease with in situ converted nigral neurons.; Authors - Hao Qian
human,mouse	WB,IHC	Cell Signal; Profile of 5-HT2A receptor involved in signaling cascades associated to intracellular inflammation and apoptosis in hepatocytes and its role in carbon tetrachloride-induced hepatotoxicity; Authors - Yuxin Zhang
human	WB	Dis Model Mech; A Matrigel-based 3D construct of SH-SY5Y cells models the α-synuclein pathologies of Parkinson's disease.; Authors - Zhao-Feng Li
mouse	IHC,IF	Toxicol Appl Pharmacol; PMID: 21703292; Authors - Fox Donald A DA
human	WB	J Transl Med; Retinal pigment epithelial cells secrete neurotrophic factors and synthesize dopamine: possible contribution to therapeutic effects of RPE cell transplantation in Parkinson's disease.; Authors - Ming Ming
human,mouse	WB,IHC	Toxicol Appl Pharmacol; Carbon tetrachloride induced mitochondrial division, respiratory chain damage, abnormal intracellular [H+] and apoptosis are due to the activation of 5-HT degradation system in hepatocytes.; Authors - Yuxin Zhang