Various lysates were subjected to SDS PAGE followed by western blot with 67568-2-Ig (STAT4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control.
Various lysates were subjected to SDS PAGE followed by western blot with 67568-2-Ig (STAT4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control.
WB analysis of 4T1 using 67568-2-Ig
4T1 cells were subjected to SDS PAGE followed by western blot with 67568-2-Ig (STAT4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
4T1 cells were subjected to SDS PAGE followed by western blot with 67568-2-Ig (STAT4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
WB analysis using 67568-2-Ig
Various lysates were subjected to SDS PAGE followed by western blot with 67568-2-Ig (STAT4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
Various lysates were subjected to SDS PAGE followed by western blot with 67568-2-Ig (STAT4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
IHC staining of human breast cancer using 67568-2-Ig
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67568-2-Ig (STAT4 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67568-2-Ig (STAT4 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human breast cancer using 67568-2-Ig
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67568-2-Ig (STAT4 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67568-2-Ig (STAT4 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human cervical cancer using 67568-2-Ig
Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 67568-2-Ig (STAT4 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 67568-2-Ig (STAT4 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human cervical cancer using 67568-2-Ig
Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 67568-2-Ig (STAT4 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 67568-2-Ig (STAT4 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF Staining of HepG2 using 67568-2-Ig
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 67568-2-Ig (STAT4 antibody), at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 67568-2-Ig (STAT4 antibody), at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).
FC experiment of HepG2 using 67568-2-Ig
1x10^6 HepG2 cells were intracellularly stained with 0.4 ug STAT4 Monoclonal antibody (67568-2-Ig, Clone:4A8C9) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).
1x10^6 HepG2 cells were intracellularly stained with 0.4 ug STAT4 Monoclonal antibody (67568-2-Ig, Clone:4A8C9) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).
The Proteintech guarantee covers Proteintech antibodies in any species and any application, including those not listed on the datasheet. If the antibody doesn’t perform, you can receive a hassle-free refund or credit note.
human breast cancer tissue, human cervical cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected in
HepG2 cells
Positive FC (Intra) detected in
HepG2 cells
Recommended dilution
Application
Dilution
Western Blot (WB)
WB : 1:5000-1:50000
Immunohistochemistry (IHC)
IHC : 1:250-1:1000
Immunofluorescence (IF)/ICC
IF/ICC : 1:50-1:500
Flow Cytometry (FC) (INTRA)
FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.
PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage Conditions
Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.
Background Information
The JAK/STAT pathway is an extensive signaling pathway downstream of cytokine receptors. STATs are cytosolic proteins with a common structure consisting of an N-terminal oligomerization domain, which favors formation of STAT dimers, followed by a DNA-binding domain and a C-terminal SRC homology-2 (SH2) domain, which is involved in association between STATs and receptors[PMID:22383755]. Signal Transducer and Activator of Transcription 4 (STAT4) is a transcription factor that is activated by IL-12 signaling and promotes Th1-cell differentiation and IFN-γ production [PMID:21998209].
STAT4-Mediated Klotho Up-Regulation Contributes to the Brain Ischemic Tolerance by Cerebral Ischemic Preconditioning via Inhibiting Neuronal Pyroptosis
Downregulated GPX4 in salivary gland epithelial cells contributes to salivary secretion dysfunction in Sjogren's syndrome via lipid ROS/pSTAT4/AQP5 axis
Various lysates were subjected to SDS PAGE followed by western blot with 67568-2-Ig (STAT4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Alpha Tubulin Monoclonal antibody (HRP-66031) as loading control.
WB analysis of 4T1 using 67568-2-Ig
4T1 cells were subjected to SDS PAGE followed by western blot with 67568-2-Ig (STAT4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
WB analysis using 67568-2-Ig
Various lysates were subjected to SDS PAGE followed by western blot with 67568-2-Ig (STAT4 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.
IHC Figures
IHC staining of human breast cancer using 67568-2-Ig
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67568-2-Ig (STAT4 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human breast cancer using 67568-2-Ig
Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67568-2-Ig (STAT4 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human cervical cancer using 67568-2-Ig
Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 67568-2-Ig (STAT4 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human cervical cancer using 67568-2-Ig
Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue slide using 67568-2-Ig (STAT4 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IF/ICC Figures
IF Staining of HepG2 using 67568-2-Ig
Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 67568-2-Ig (STAT4 antibody), at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).
FC (INTRA) Figures
FC experiment of HepG2 using 67568-2-Ig
1x10^6 HepG2 cells were intracellularly stained with 0.4 ug STAT4 Monoclonal antibody (67568-2-Ig, Clone:4A8C9) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1)(red), or 0.4 ug Mouse IgG1 Isotype Control (MOPC-21) (65124-1-Ig, Clone: MOPC-21) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).
The species listed in Tested Reactivity are in-house verified and applicable species. For unlisted species, please refer to the homology analysis of the immunogen sequence and related species. For rabbit polyclonal antibodies, homology >70% is recommended. For mouse monoclonal antibodies and rabbit recombinant antibodies, homology >90% is recommended. Generally, the higher the homology, the greater the applicability. However, there will be certain differences in protein expression in different species, tissues or cells. Therefore, the homology analysis results are for reference only and do not serve as a guarantee.
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Proteintech
STAT4 Monoclonal antibody
Catalog Number
67568-2-Ig
Citations
2
Dilutions
WB : 1:5000-1:50000 IHC : 1:250-1:1000 IF/ICC : 1:50-1:500 FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
Applications
WB, IHC, IF/ICC, FC (Intra), ELISA
Reactivity
human, mouse, rat
Product Guarantee
Covers any species including not listed on datasheet
Covers any applications including not listed on datasheet
