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CoraLite® Plus 488-conjugated SMARCA4 Monoclonal antibody

SMARCA4 Monoclonal Antibody for IF/ICC

Host / Isotype

Mouse / IgG2b

Reactivity

Human

Applications

IF/ICC

Conjugate

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

2E6B6

Cat no : CL488-66561

Synonyms

ATP dependent helicase SMARCA4, BAF190, BAF190A, BRG1, BRG1 associated factor 190A, FLJ39786, hSNF2b, Protein brahma homolog 1, Protein BRG 1, SMARCA4, SNF2, SNF2 BETA, SNF2B, SNF2L4, SNF2LB, SWI2, Transcription activator BRG1

Validation Data Gallery

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  • Immunofluorescence (IF) / fluorescent staining of HepG2 cells using CoraLite® Plus 488-conjugated SMARCA4 Monoclonal a (CL488-66561)

    IF Staining of HepG2 using CL488-66561

    Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CL488-66561 (SMARCA4 antibody) at dilution of 1:50.

Tested Applications

Positive IF/ICC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

CL488-66561 targets SMARCA4 in IF/ICC applications and shows reactivity with Human samples.

Tested Reactivity Human
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen SMARCA4 fusion protein Ag16256
Full Name SWI/SNF related, matrix associated, actin dependent regulator of chromatin, subfamily a, member 4
Calculated Molecular Weight 1647 aa, 185 kDa
GenBank Accession NumberBC150298
Gene Symbol SMARCA4
Gene ID (NCBI) 6597
RRIDAB_2883355
Conjugate CoraLite® Plus 488 Fluorescent Dye
Excitation/Emission Maxima Wavelengths493 nm / 522 nm
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
Storage ConditionsStore at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

SMARCA4, also named as BAF190A, BRG1, SNF2B and SNF2L4, belongs to the SNF2/RAD54 helicase family. SMARCA4 is a transcriptional coactivator cooperating with nuclear hormone receptors to potentiate transcriptional activation. It is a component of the CREST-BRG1 complex, a multiprotein complex that regulates promoter activation by orchestrating a calcium-dependent release of a repressor complex and a recruitment of an activator complex. It is also involved in vitamin D-coupled transcription regulation via its association with the WINAC complex, a chromatin-remodeling complex recruited by vitamin D receptor (VDR), which is required for the ligand-bound VDR-mediated transrepression of the CYP27B1 gene. And this anitbody is CL488（Ex/Em 488 nm/515 nm） conjugated.

Protocols

Product Specific Protocols
IF protocol for CL Plus 488 SMARCA4 antibody CL488-66561Download protocol
Standard Protocols
Click here to view our Standard Protocols