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  • KD/KO Validated

MYOD1 Polyclonal antibody

MYOD1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (7)

Applications

WB, IHC, IF, ChIP, ELISA

Conjugate

Unconjugated

Cat no : 18943-1-AP

Synonyms

bHLHc1, Myf 3, MYF3, MYOD, MYOD1, myogenic differentiation 1, Myogenic factor 3, PUM



Tested Applications

Positive WB detected inmouse heart tissue, human heart tissue, mouse skeletal muscle tissue, rat heart tissue
Positive IHC detected inmouse embryo tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

18943-1-AP targets MYOD1 in WB, IHC, IF, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, pig, rabbit, chicken, zebrafish, sheep, goat, duck
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MYOD1 fusion protein Ag13512
Full Name myogenic differentiation 1
Calculated Molecular Weight 320 aa, 35 kDa
Observed Molecular Weight 35-45 kDa
GenBank Accession NumberBC064493
Gene Symbol MYOD1
Gene ID (NCBI) 4654
RRIDAB_10603467
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MYOD1, also named as BHLHC1 or MYF3, is a 320 amino acid protein, which promotes the transcriptional activity of MYOD1 through its CDK9-mediated phosphorylation. This phosphorylation promotes its function in muscle differentiation. MYOD1 acts as a transcriptional activator that promotes transcription of muscle-specific target genes and plays a role in muscle differentiation. MYOD1 together with MYF5 and MYOG, co-occupies muscle-specific gene promoter core region during myogenesis. Induces fibroblasts to differentiate into myoblasts. he calcualted molecular weight of MYOD1 is 34 kDa, but modified MYOD1 is about 45 kDa. (PMID: 12037670)

Protocols

Product Specific Protocols
WB protocol for MYOD1 antibody 18943-1-APDownload protocol
IHC protocol for MYOD1 antibody 18943-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Nature

A plant-derived natural photosynthetic system for improving cell anabolism

Authors - Pengfei Chen
mouseIHC

Nature

Divergent transcriptional regulation of astrocyte reactivity across disorders.

Authors - Joshua E Burda
WB

Nature

SAR1B senses leucine levels to regulate mTORC1 signalling.

Authors - Jie Chen
ratIF

Sci Adv

Bioactive fiber-reinforced hydrogel to tailor cell microenvironment for structural and functional regeneration of myotendinous junction

Authors - Yuzhi Sun
mouseWB

Nat Commun

The transcription factor NF-Y participates to stem cell fate decision and regeneration in adult skeletal muscle.

Authors - Giovanna Rigillo
mouseWB

J Cachexia Sarcopenia Muscle

circRNAome profiling reveals circFgfr2 regulates myogenesis and muscle regeneration via a feedback loop.

Authors - Junyu Yan

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

Charlotte (Verified Customer) (07-23-2024)

I reveal MyoD1 in C2C12 cells that differentiate into myoblast lineage upon stimulation. Antibodies were diluted in 5% milk TBST. The upper band is the expected one. I guess the lower one (< 35kDa) is aspecific

  • Applications: Western Blot
  • Primary Antibody Dilution: 1/1000
  • Cell Tissue Type: C2C12
MYOD1 Antibody Western Blot validation (1/1000 dilution) in C2C12 (Cat no:18943-1-AP)
FH

Cassandra (Verified Customer) (02-04-2021)

This antibody worked quite well in my IF staining. I used 1 to 100 antibody dilution.

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:100
  • Cell Tissue Type: Satellite cell
MYOD1 Antibody Immunofluorescence validation (1:100 dilution) in Satellite cell (Cat no:18943-1-AP)