E2A Polyclonal antibody

• Featured Product
• KD/KO Validated

E2A Polyclonal Antibody for IF, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, CoIP, ELISA

Conjugate

Unconjugated

Publications

5

Cat no : 21242-1-AP

Synonyms

bHLHb21, E2A, E47, ITF1, Kappa E2 binding factor, TCF 3, TCF3, Transcription factor 3, Transcription factor E2 alpha, Transcription factor ITF 1

---

Validation Data Gallery

• 

  WB analysis of Jurkat using 21242-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 21242-1-AP (E2A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 21242-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21242-1-AP (E2A antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 21242-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 21242-1-AP (E2A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 21242-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 21242-1-AP (E2A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of Jurkat using 21242-1-AP

  IP Result of anti-E2A (IP:21242-1-AP, 4ug; Detection:21242-1-AP 1:500) with Jurkat cells lysate 2800ug.

• 

  IF Staining of HepG2 using 21242-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 21242-1-AP (E2A antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

---

Tested Applications

• Positive WB detected in: Jurkat cells, PC-3 cells, K-562 cells, NIH/3T3 cells
• Positive IP detected in: Jurkat cells
• Positive IF detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB
• Immunofluorescence (IF): IF : 1:50-1:500

Sample-dependent, check data in validation data gallery

Published Applications

• KD/KO: See 1 publications below
• WB: See 4 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Product Information

The immunogen of 21242-1-AP is E2A Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: E2A fusion protein Ag15734
• Full Name: transcription factor 3 (E2A immunoglobulin enhancer binding factors E12/E47)
• Calculated molecular weight: 654 aa, 68 kDa
• Observed molecular weight: 68 kDa
• GenBank accession number: BC110580
• Gene symbol: TCF3
• Gene ID (NCBI): 6929
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB,CoIP	Cell Signal; Targeting SDCBP2 in acute myeloid leukemia; Authors - Yan Du
human	WB	J Cell Mol Med; The role of E2A in ATPR-induced cell differentiation and cycle arrest in acute myeloid leukaemia cells.; Authors - Meiju Zhang ; KD Validated
mouse	WB	Mol Immunol; Ablation of core fucosylation attenuates the signal transduction via T cell receptor to suppress the T cell development.; Authors - Wei Liang
rat	IP	Life Sci; miR-27a promotes endothelial-mesenchymal transition in hypoxia-induced pulmonary arterial hypertension by suppressing BMP signaling.; Authors - Ting Liu
mouse	WB	Nat Commun; A p38α-BLIMP1 signalling pathway is essential for plasma cell differentiation; Authors - Jianfeng Wu