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- KD/KO Validated
E2A Polyclonal antibody
E2A Polyclonal Antibody for IF, IP, WB, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IP, IF, CoIP, ELISA
Conjugate
Unconjugated
Cat no : 21242-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | Jurkat cells, PC-3 cells, K-562 cells, NIH/3T3 cells |
Positive IP detected in | Jurkat cells |
Positive IF detected in | HepG2 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:3000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB |
Immunofluorescence (IF) | IF : 1:50-1:500 |
Sample-dependent, check data in validation data gallery |
Published Applications
KD/KO | See 1 publications below |
WB | See 4 publications below |
IP | See 1 publications below |
CoIP | See 1 publications below |
Product Information
The immunogen of 21242-1-AP is E2A Fusion Protein expressed in E. coli.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human, mouse, rat |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | E2A fusion protein Ag15734 |
Full Name | transcription factor 3 (E2A immunoglobulin enhancer binding factors E12/E47) |
Calculated molecular weight | 654 aa, 68 kDa |
Observed molecular weight | 68 kDa |
GenBank accession number | BC110580 |
Gene symbol | TCF3 |
Gene ID (NCBI) | 6929 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
Product Specific Protocols | |
---|---|
WB protocol for E2A antibody 21242-1-AP | Download protocol |
IF protocol for E2A antibody 21242-1-AP | Download protocol |
IP protocol for E2A antibody 21242-1-AP | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
J Cell Mol Med The role of E2A in ATPR-induced cell differentiation and cycle arrest in acute myeloid leukaemia cells.
| ||
Mol Immunol Ablation of core fucosylation attenuates the signal transduction via T cell receptor to suppress the T cell development. | ||
Life Sci miR-27a promotes endothelial-mesenchymal transition in hypoxia-induced pulmonary arterial hypertension by suppressing BMP signaling. | ||