MYF6 Polyclonal antibody
MYF6 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IF/ICC, chip, ELISA
Conjugate
Unconjugated
Cat no : 11754-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | mouse skeletal muscle tissue |
Positive IHC detected in | mouse skeletal muscle tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF/ICC detected in | C2C12 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:1000 |
Immunohistochemistry (IHC) | IHC : 1:500-1:2000 |
Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
WB | See 2 publications below |
ChIP | See 1 publications below |
Product Information
11754-1-AP targets MYF6 in WB, IHC, IF/ICC, chip, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | MYF6 fusion protein Ag2350 |
Full Name | myogenic factor 6 (herculin) |
Calculated Molecular Weight | 242 aa, 27 kDa |
Observed Molecular Weight | 27 kDa |
GenBank Accession Number | BC017834 |
Gene Symbol | MYF6 |
Gene ID (NCBI) | 4618 |
RRID | AB_2250929 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
Product Specific Protocols | |
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WB protocol for MYF6 antibody 11754-1-AP | Download protocol |
IHC protocol for MYF6 antibody 11754-1-AP | Download protocol |
IF protocol for MYF6 antibody 11754-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Nat Commun MRF4 negatively regulates adult skeletal muscle growth by repressing MEF2 activity. | ||
Front Cell Dev Biol A Functional SNP in the Promoter of LBX1 Is Associated With the Development of Adolescent Idiopathic Scoliosis Through Involvement in the Myogenesis of Paraspinal Muscles. | ||
In Vitro Cell Dev Biol Anim Expression patterns and correlation analyses of muscle-specific genes in the process of sheep myoblast differentiation |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Kenzo (Verified Customer) (11-11-2023) | Worked well for frozen mouse heart tissue sections.
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