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MAD2L2 Monoclonal antibody

MAD2L2 Monoclonal Antibody for WB, IHC, FC (Intra), ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat

Applications

WB, IHC, FC (Intra), ELISA

Conjugate

Unconjugated

CloneNo.

2A4C2

Cat no : 67100-1-Ig

Synonyms

Mitotic arrest deficient 2-like protein 2, MAD2-like protein 2, MAD2B, MAD2 like protein 2, hREV7

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using MAD2L2 Monoclonal antibody (67100-1-Ig)

    WB analysis using 67100-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67100-1-Ig (MAD2L2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of various lysates using MAD2L2 Monoclonal antibody (67100-1-Ig)

    WB analysis using 67100-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 67100-1-Ig (MAD2L2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human lymphoma tissue using MAD2L2 Monoclonal antibody (67100-1-Ig)

    IHC staining of human lymphoma using 67100-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 67100-1-Ig (MAD2L2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human lymphoma tissue using MAD2L2 Monoclonal antibody (67100-1-Ig)

    IHC staining of human lymphoma using 67100-1-Ig

    Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 67100-1-Ig (MAD2L2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Flow cytometry (FC) experiment of K-562 cells using MAD2L2 Monoclonal antibody (67100-1-Ig)

    FC experiment of K-562 using 67100-1-Ig

    1X10^6 K-562 cells were intracellularly stained with 0.4 ug Anti-Human MAD2L2 (67100-1-Ig, Clone:2A4C2) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2b Isotype Control (MPC-11) (65128-1-Ig, Clone: MPC-11) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

Tested Applications

Positive WB detected inHeLa cells, HEK-293 cells, MCF-7 cells, Jurkat cells, HSC-T6 cells, NIH/3T3 cells, 4T1 cells
Positive IHC detected inhuman lymphoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive FC (Intra) detected inK-562 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:20000
Immunohistochemistry (IHC)IHC : 1:500-1:2000
Flow Cytometry (FC) (INTRA)FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67100-1-Ig targets MAD2L2 in WB, IHC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Mouse / IgG2b
Class Monoclonal
Type Antibody
Immunogen MAD2L2 fusion protein Ag28233
Full Name MAD2 mitotic arrest deficient-like 2 (yeast)
Calculated Molecular Weight 211 aa, 24 kDa
Observed Molecular Weight 24 kDa
GenBank Accession NumberBC015244
Gene Symbol MAD2L2
Gene ID (NCBI) 10459
RRIDAB_2882405
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MAD family, together with BUB and Mps1,Cdc20k, play roles in the mitotic spindle checkpoint. MAD2L2 is one of the MAD family. It can mediate the second polymerase switching in translation DNA synthesis by mediating the interaction between the error-prone DNA polymerase zeta catalytic subunit REV3L and the inserter polymerase REV1. Through regulation of the JNK-mediate phosphorylation and activation of the transcriptional activator ELK1, MAD2L2 involves in cellular response to DNA damage. Also it has role in the progression of cell cycle and peithelial-mesenchymal transdifferentiation.

Protocols

Product Specific Protocols
WB protocol for MAD2L2 antibody 67100-1-IgDownload protocol
IHC protocol for MAD2L2 antibody 67100-1-IgDownload protocol
FC protocol for MAD2L2 antibody 67100-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols