MAD2L2 Monoclonal antibody, PBS Only

MAD2L2 Monoclonal Antibody for Indirect ELISA, IHC, WB

Host / Isotype

Mouse / IgG2b

Reactivity

rat, mouse, human

Applications

Indirect ELISA, IHC, WB

Conjugate

Unconjugated

CloneNo.

2A4C2

Cat no : 67100-1-PBS

Synonyms

Mitotic arrest deficient 2-like protein 2, MAD2-like protein 2, MAD2B, MAD2 like protein 2, hREV7

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Validation Data Gallery

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  WB analysis using 67100-1-Ig (same clone as 67100-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67100-1-Ig (MAD2L2 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67100-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 67100-1-Ig (same clone as 67100-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67100-1-Ig (MAD2L2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67100-1-PBS in a different storage buffer formulation.

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  IHC staining of human lymphoma using 67100-1-Ig (same clone as 67100-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 67100-1-Ig (MAD2L2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67100-1-PBS in a different storage buffer formulation.

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  IHC staining of human lymphoma using 67100-1-Ig (same clone as 67100-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 67100-1-Ig (MAD2L2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67100-1-PBS in a different storage buffer formulation.

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  FC experiment of K-562 using 67100-1-Ig (same clone as 67100-1-PBS)

  1X10^6 K-562 cells were intracellularly stained with 0.4 ug Anti-Human MAD2L2 (67100-1-Ig, Clone:2A4C2) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2b Isotype Control (MPC-11) (65128-1-Ig, Clone: MPC-11) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011). This data was developed using the same antibody clone with 67100-1-PBS in a different storage buffer formulation.

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Product Information

67100-1-PBS targets MAD2L2 in Indirect ELISA, IHC, WB applications and shows reactivity with rat, mouse, human samples.

• Tested Reactivity: rat, mouse, human
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: MAD2L2 fusion protein Ag28233
• Full Name: MAD2 mitotic arrest deficient-like 2 (yeast)
• Calculated Molecular Weight: 211 aa, 24 kDa
• Observed Molecular Weight: 24 kDa
• GenBank Accession Number: BC015244
• Gene Symbol: MAD2L2
• Gene ID (NCBI): 10459
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

MAD family, together with BUB and Mps1,Cdc20k, play roles in the mitotic spindle checkpoint. MAD2L2 is one of the MAD family. It can mediate the second polymerase switching in translation DNA synthesis by mediating the interaction between the error-prone DNA polymerase zeta catalytic subunit REV3L and the inserter polymerase REV1. Through regulation of the JNK-mediate phosphorylation and activation of the transcriptional activator ELK1, MAD2L2 involves in cellular response to DNA damage. Also it has role in the progression of cell cycle and peithelial-mesenchymal transdifferentiation.