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  • Featured Product
  • KD/KO Validated

IFT88 Polyclonal antibody

IFT88 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, canine and More (3)

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Conjugate

Unconjugated

Cat no : 13967-1-AP

Synonyms

TTC10, TG737, Intraflagellar transport protein 88 homolog

Validation Data Gallery

Filter:
  • Western Blot (WB) analysis of various lysates using IFT88 Polyclonal antibody (13967-1-AP)

    WB analysis using 13967-1-AP

    Various lysates were subjected to SDS PAGE followed by western blot with 13967-1-AP (IFT88 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of knockout cells and WT cells using IFT88 Polyclonal antibody (13967-1-AP)

    IP experiment of knockout cells and WT cells using 13967-1-AP

    IP and WB result of IFT88 (13967-1-AP) from Dr. Corbit, Kevin. Knockout cells and WT cells.

  • Immunoprecipitation (IP) experiment of HEK-293 cells using IFT88 Polyclonal antibody (13967-1-AP)

    IP experiment of HEK-293 using 13967-1-AP

    IP result of anti-IFT88 (IP:13967-1-AP, 3ug; Detection:13967-1-AP 1:800) with HEK-293 cells lysate 1500ug.

  • Immunohistochemistry (IHC) staining of rat heart tissue using IFT88 Polyclonal antibody (13967-1-AP)

    IHC staining of rat heart using 13967-1-AP

    Immunohistochemical analysis of paraffin-embedded rat heart tissue slide using 13967-1-AP (IFT88 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of mouse heart tissue using IFT88 Polyclonal antibody (13967-1-AP)

    IHC staining of mouse heart using 13967-1-AP

    Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 13967-1-AP (IFT88 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • Immunohistochemistry (IHC) staining of human pancreas tissue using IFT88 Polyclonal antibody (13967-1-AP)

    IHC staining of human pancreas using 13967-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas using 13967-1-AP (IFT88 antibody) at dilution of 1:100 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human pancreas tissue using IFT88 Polyclonal antibody (13967-1-AP)

    IHC staining of human pancreas using 13967-1-AP

    Immunohistochemical analysis of paraffin-embedded human pancreas using 13967-1-AP (IFT88 antibody) at dilution of 1:100 (under 10x lens).

  • Immunofluorescence (IF) / fluorescent staining of hTERT-RPE1 cells using IFT88 Polyclonal antibody (13967-1-AP)

    IF Staining of hTERT-RPE1 using 13967-1-AP

    Immunofluorescent analysis of (4% PFA) fixed hTERT-RPE1 cells using IFT88 antibody (13967-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of C2C12 cells using IFT88 Polyclonal antibody (13967-1-AP)

    IF Staining of C2C12 using 13967-1-AP

    Immunofluorescent analysis of (4% PFA) fixed C2C12 cells using IFT88 antibody (13967-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of C2C12 cells using IFT88 Polyclonal antibody (13967-1-AP)

    IF Staining of C2C12 using 13967-1-AP

    Immunofluorescent analysis of (4% PFA) fixed C2C12 cells using IFT88 antibody (13967-1-AP) at dilution of 1:200 and CoraLite®594-Conjugated Goat Anti-Rabbit IgG(H+L), acetylated Tubulin(Lys40) antibody (66200-1-Ig, Clone: 7E5H8, green).

  • Immunofluorescence (IF) / fluorescent staining of hTERT-RPE1 cells using IFT88 Polyclonal antibody (13967-1-AP)

    IF Staining of hTERT-RPE1 using 13967-1-AP

    Immunofluorescent analysis of (4% PFA) fixed hTERT-RPE1 cells using IFT88 antibody (13967-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

  • Immunofluorescence (IF) / fluorescent staining of MDCK cells using IFT88 Polyclonal antibody (13967-1-AP)

    IF Staining of MDCK using 13967-1-AP

    Immunofluorescent analysis of (4% PFA) fixed MDCK cells using IFT88 antibody (13967-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002).

  • Immunofluorescence (IF) / fluorescent staining of MDCK cells using IFT88 Polyclonal antibody (13967-1-AP)

    IF Staining of MDCK using 13967-1-AP

    Immunofluorescent images of MDCK cells stained with IFT88 rabbit pAb (13967-1-AP) and acetylated tubulin mouse mAb (66200-1-Ig) at dilution of 1:50, further stained with Alexa Fluor 594-conjugated Goat Anti-Rabbit IgG(H+L) for 13967-1-AP, and Alexa Fluor 488-conjugated Goat anti-Mouse IgG(H+L) for 66200-1-Ig.

Tested Applications

Positive WB detected inHEK-293 cells, NIH/3T3 cell, MDCK cells, mouse thymus tissue
Positive IP detected inknockout cells and WT cells, HEK-293 cells
Positive IHC detected inmouse heart tissue, human pancreas tissue, rat heart tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF/ICC detected inhTERT-RPE1 cells, MDCK cells, C2C12 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:12000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:200-1:800
Immunofluorescence (IF)/ICCIF/ICC : 1:200-1:800
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

13967-1-AP targets IFT88 in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat, canine samples.

Tested Reactivity human, mouse, rat, canine
Cited Reactivityhuman, mouse, rat, pig, canine, chicken, zebrafish
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen IFT88 fusion protein Ag4980
Full Name intraflagellar transport 88 homolog (Chlamydomonas)
Calculated Molecular Weight 94 kDa
Observed Molecular Weight94 kDa
GenBank Accession NumberBC030776
Gene Symbol IFT88
Gene ID (NCBI) 8100
RRIDAB_2121979
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Intraflagellar transport (IFT), mediated by molecular motors and IFT particles, is an important transport process that occurs in the cilium and has been shown to be essential for the assembly and maintenance of cilia and flagella in many organisms. IFT88 (intraflagellar transport protein 88; also known as TG737 or TTC10) is a component of IFT particles and required for cilium biogenesis. Defects in IFT88/Tg737 lead to polycystic kidney disease (11062270). IFT88 localizes to spindle poles during mitosis and is required for spindle orientation in mitosis (21441926). This antibody was raised against the C-terminal region of human IFT88 and can detect the endogenous level of IFT88.

Protocols

Product Specific Protocols
WB protocol for IFT88 antibody 13967-1-APDownload protocol
IHC protocol for IFT88 antibody 13967-1-APDownload protocol
IF protocol for IFT88 antibody 13967-1-APDownload protocol
IP protocol for IFT88 antibody 13967-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIF

Nature

Small-molecule inhibitors of the AAA+ ATPase motor cytoplasmic dynein.

Authors - Firestone Ari J AJ
View Article
mouseWB

Nature

Functional interaction between autophagy and ciliogenesis.

Authors - Pampliega Olatz O
View Article
chickenIF

Science

Apical abscission alters cell polarity and dismantles the primary cilium during neurogenesis.

Authors - Raman M Das
View Article
mouseIF

Nat Genet

A transition zone complex regulates mammalian ciliogenesis and ciliary membrane composition.

Authors - Garcia-Gonzalo Francesc R FR
View Article
mouseIF

Nat Genet

Complex interactions between genes controlling trafficking in primary cilia.

Authors - Ocbina Polloneal Jymmiel R PJ
View Article
humanIF

Nat Genet

Mutations in PLK4, encoding a master regulator of centriole biogenesis, cause microcephaly, growth failure and retinopathy.

Authors - Carol-Anne Martin
View Article

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

FH

Parijat (Verified Customer) (04-08-2024)

Good Antibody for IF.

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:100
FH

Serhiy (Verified Customer) (01-04-2023)

This antibody detects Ift88 in ciliary axonemes of immunofluorescently stained IMCD3 cells (atteched image: red Ift88; green - gamma-tubulin) and mouse fibroblasts. We also tried to detect endogenous Ift88 in NIH3T3 cells by western blots and found that antibody detects several non-specific bands in addition to apparently specific one between the 75-100 kDa markers.

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:100 (IF), 1:1000 (WB)
  • Cell Tissue Type: NIH3T3 fibroblasts, IMCD3 kidney collecting duct cells
IFT88 Antibody Immunofluorescence validation (1:100 (IF), 1:1000 (WB) dilution) in NIH3T3 fibroblasts, IMCD3 kidney collecting duct cells (Cat no:13967-1-AP)
FH

Charlotte (Verified Customer) (07-26-2022)

We have revealed IFT88 after stripping a couple of times. The signal remains nice and the unspecific bands might be due to lower stripping efficiency

  • Applications: Western Blot, Immunofluorescence
  • Primary Antibody Dilution: 1/500
  • Cell Tissue Type: NIH-3T3
IFT88 Antibody Western Blot,Immunofluorescence validation (1/500 dilution) in NIH-3T3 (Cat no:13967-1-AP)
FH

Stephen (Verified Customer) (02-08-2022)

works well in RPE cells stains Primary cilia seen by co-staining with Ac-Tubulin

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: Human RPE-1 cells
IFT88 Antibody Immunofluorescence validation (1:500 dilution) in Human RPE-1 cells (Cat no:13967-1-AP)
FH

Boyan (Verified Customer) (01-31-2019)

Excellent. Works quite well both in WB and IF.

  • Applications: Western Blot, Immunofluorescence,