- Featured Product
- KD/KO Validated
IFITM1-Specific Monoclonal antibody
IFITM1-Specific Monoclonal Antibody for WB, IHC, IF/ICC, IP, ELISA
Host / Isotype
Mouse / IgG2a
Reactivity
human and More (1)
Applications
WB, IHC, IF/ICC, IP, CoIP, ELISA
Conjugate
Unconjugated
CloneNo.
5B5E2
Cat no : 60074-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | TF-1 cells, K-562 cells, human testis tissue, HepG2 cells, HL-60 cells |
| Positive IP detected in | HepG2 cells |
| Positive IHC detected in | human lymphoma tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | K-562 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:20000-1:100000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:200-1:1000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
60074-1-Ig targets IFITM1-Specific in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | IFITM1-Specific fusion protein Ag2320 |
| Full Name | interferon induced transmembrane protein 1 (9-27) |
| Calculated Molecular Weight | 14 kDa |
| Observed Molecular Weight | 14-17 kDa |
| GenBank Accession Number | BC000897 |
| Gene Symbol | IFITM1 |
| Gene ID (NCBI) | 8519 |
| RRID | AB_2233405 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS with 0.1% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
IFITM1(interferon induced transmembrane protein), also named DSPA2a and interferon-induced protein 17 (IFI17), belongs to the CD225 family. It has two transmembrane domain and serves as an IFN-induced antiviral protein that mediates cellular innate immunity to at least three major human pathogens, influenza A H1N1 virus, West Nile virus (WNV), and dengue virus , by inhibiting the early steps of replication. IFITM proteins are recently identified as viral restriction factors that inhibit infection mediated by the influenza A virus (IAV) hemagglutinin (HA) protein. Also they serve as important components of the innate immune system to restrict HIV-1 infection. 60074-1-Ig is a mouse monoclonal antibody which specifically recognizing IFITM1 but not IFITM2 or IFITM3.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for IFITM1-Specific antibody 60074-1-Ig | Download protocol |
| IHC protocol for IFITM1-Specific antibody 60074-1-Ig | Download protocol |
| IF protocol for IFITM1-Specific antibody 60074-1-Ig | Download protocol |
| IP protocol for IFITM1-Specific antibody 60074-1-Ig | Download protocol |
| FC protocol for IFITM1-Specific antibody 60074-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Science IFITM proteins inhibit placental syncytiotrophoblast formation and promote fetal demise. | ||
Cell The IFITM proteins mediate cellular resistance to influenza A H1N1 virus, West Nile virus, and dengue virus. | ||
Nat Microbiol Interferon-induced transmembrane protein-1 competitively blocks Ephrin receptor A2-mediated Epstein-Barr virus entry into epithelial cells | ||
Sci Transl Med Dimethyl fumarate potentiates oncolytic virotherapy through NF-κB inhibition. | ||
Cell Host Microbe Resistance of Transmitted Founder HIV-1 to IFITM-Mediated Restriction.
|
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Claudia (Verified Customer) (06-20-2022) | The antibody worked very well for the analysis of IFITM1 expression in permanent cell lines.
|
