Tested Applications
| Positive WB detected in | Recombinant protein |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
HRP-66002 targets GFP tag in WB, IF, CoIP applications and shows reactivity with aequorea victoria, recombinant protein samples.
| Tested Reactivity | aequorea victoria, recombinant protein |
| Cited Reactivity | human, bovine |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
Recombinant protein Predict reactive species |
| Full Name | GFP tag |
| Calculated Molecular Weight | 26 kDa |
| Observed Molecular Weight | - |
| GenBank Accession Number | M62653 |
| Gene Symbol | |
| Gene ID (NCBI) | |
| RRID | AB_2883834 |
| Conjugate | HRP |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P42212 |
| Storage Buffer | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Green Fluorescent Proteins (GFPs) encompass a diverse range of proteins carrying a green chromophore, originating from various species and forming different protein lineages.
Wildtype GFP consists of 238 amino acid residues (26.9 kDa). GFP was first identified in the jellyfish Aequorea victoria. It emits green light with a peak wavelength of 509 nm upon excitation by blue light at 395 nm.
When fused with other proteins, GFP serves as a versatile reporter protein e.g. for quantifying expression levels or facilitates visualization of subcellular localization through fluorescence microscopy.
This antibody is a HRP conjugated mouse (IgG2a) monoclonal antibody against GFP reactive to GFP, eGFP, eYFP, YFP and CFP.
Note: Do not add Azium (Sodium Azide or Smite) into the dilution buffer. Azium is the HRP inhibitor which decreases the enzymatic activity of HRP.
Publications
What published studies show
The HRP-conjugated GFP tag Monoclonal antibody (HRP-66002) has 28 citations. These appear in journals including Cell, Nature Communications, Molecular Cell, Journal of Clinical Investigation, Plant Cell, PNAS, eLife, PLoS Biology, Cell Death & Disease, and Frontiers in Microbiology. Research fields span immunology, Alzheimer's disease and tauopathy, cancer biology, plant biology, virology, lipid droplet biology, and cell signaling. Most citations use the antibody for Western blot, with some for immunofluorescence and co-immunoprecipitation.
| Species | Application | Title |
|---|---|---|
Cell Human T-bet Governs Innate and Innate-like Adaptive IFN-γ Immunity against Mycobacteria. | ||
Mol Neurodegener Islet amyloid polypeptide cross-seeds tau and drives the neurofibrillary pathology in Alzheimer's disease. | ||
Nat Commun Molecular determinants for the association of human hormone-sensitive lipase with lipid droplets | ||
Nat Commun NEDAMSS syndrome-related truncating and missense mutations are associated with aberrant liquid-liquid phase separation of IRF2BPL. | ||
Nat Commun Nuclear ANLN regulates transcription initiation related Pol II clustering and target gene expression | ||
Nat Commun Boosting energy metabolism and biosynthesis in diverse organisms by a common bacterial salvage lipoylation protein. |
Reviews
What customers say
Both reviewers rated this product 5 stars. One user found it effective for detecting GFP-tagged proteins in Arabidopsis at a 1:2000 dilution for Western Blot. Another user reported it worked well at a 1:10000 dilution on HEK cells for Western Blot. Overall, customers are satisfied with its performance in WB applications across different species and dilutions.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Wei (Verified Customer) (09-11-2023) | It works well in our detection of GFP-tagged protein from Arabidopsis
|
Aamir (Verified Customer) (01-08-2020) | Worked fine for WB
|
