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- KD/KO Validated
Cyclin D1 Monoclonal antibody
Cyclin D1 Monoclonal Antibody for WB, ELISA
Host / Isotype
Mouse / IgG2b
Reactivity
human, mouse, rat and More (5)
Applications
WB, IF, IP, ELISA
Conjugate
Unconjugated
715
CloneNo.
2G3G5
Cat no : 60186-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
| Positive WB detected in | U2OS cells, A549 cells, HeLa cells, MCF-7 cells, NIH/3T3 cells, RAW 264.7 cells, SH-SY5Y cells, A431 cells, SW 1990 cells, HepG2 cells, HSC-T6 cells, PC-12 cells, HCT 116 cells, SK-N-SH cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:20000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 696 publications below |
| IF | See 10 publications below |
| IP | See 3 publications below |
Product Information
60186-1-Ig targets Cyclin D1 in WB, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rabbit, zebrafish, bovine, hamster, goat |
| Host / Isotype | Mouse / IgG2b |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen | Cyclin D1 fusion protein Ag0689 |
| Full Name | cyclin D1 |
| Calculated Molecular Weight | 295 aa, 34 kDa |
| Observed Molecular Weight | 34 kDa |
| GenBank Accession Number | BC000076 |
| Gene Symbol | Cyclin D1 |
| Gene ID (NCBI) | 595 |
| RRID | AB_10793718 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
CCND1 (cyclin D1), also known as PRAD1 or BCL1, belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance throughout the cell cycle. CCND1 forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activity is required for cell cycle G1/S transition. The CCND1 gene, located on 11q13 has been reported to be overexpressed in mantle cell lymphoma (MCL) due to the chromosomal translocation. CCND1 has been shown to interact with tumor suppressor protein Rb and the expression of this gene is regulated positively by Rb. Over-expression of CCND1 is known to correlate with the early onset of cancer and risk of tumor progression and metastasis.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for Cyclin D1 antibody 60186-1-Ig | Download protocol |
| FC protocol for Cyclin D1 antibody 60186-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Nat Cell Biol TRIM15 and CYLD regulate ERK activation via lysine-63-linked polyubiquitination. | ||
Bioact Mater Bioinspired engineering ADSC nanovesicles thermosensitive hydrogel enhance autophagy of dermal papilla cells for androgenetic alopecia treatment | ||
Adv Sci (Weinh) Inflammatory Fibroblast-Like Synoviocyte-Derived Exosomes Aggravate Osteoarthritis via Enhancing Macrophage Glycolysis | ||
Acta Pharm Sin B The substitution of SERCA2 redox cysteine 674 promotes pulmonary vascular remodeling by activating IRE1α/XBP1s pathway. | ||
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Janardhan (Verified Customer) (08-22-2020) | Antibody works ok, needs longer exposure
|
FH Yuan (Verified Customer) (01-23-2020) | The antibody is ok. Signal is not very strong for western blot. Needed relative long exposure for Hela cells.
|
FH Kyosuke (Verified Customer) (06-12-2019) | I use this as a proliferation marker and this antibody works very well for WB.
|
