Cyclin D1 Monoclonal antibody, PBS Only

• Featured Product
• KD/KO Validated

Cyclin D1 Monoclonal Antibody for WB, FC, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat

Applications

WB, FC, ELISA

Conjugate

Unconjugated

CloneNo.

2G3G5

Cat no : 60186-1-PBS

Synonyms

B cell lymphoma 1 protein, CCND1, cyclin D, cyclin D1, D11S287E, G1/S specific cyclin D1, PRAD1, PRAD1 oncogene, U21B31

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Validation Data Gallery

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  WB analysis using 60186-1-Ig (same clone as 60186-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 60186-1-Ig (Cyclin D1 antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60186-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 60186-1-Ig (same clone as 60186-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 60186-1-Ig (Cyclin D1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60186-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 60186-1-Ig (same clone as 60186-1-PBS)

  WB result of Cyclin D1 antibody (60186-1-Ig, 1:12,000) with si-Control and si-Cyclin D1 transfected HeLa cells. This data was developed using the same antibody clone with 60186-1-PBS in a different storage buffer formulation.

• 

  WB analysis of RAW 264.7 using 60186-1-Ig (same clone as 60186-1-PBS)

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 60186-1-Ig (Cyclin D1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60186-1-PBS in a different storage buffer formulation.

• 

  WB analysis of MCF-7 using 60186-1-Ig (same clone as 60186-1-PBS)

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 60186-1-Ig (Cyclin D1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60186-1-PBS in a different storage buffer formulation.

• 

  WB analysis of SH-SY5Y using 60186-1-Ig (same clone as 60186-1-PBS)

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 60186-1-Ig (CCND1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60186-1-PBS in a different storage buffer formulation.

• 

  WB analysis of NIH/3T3 using 60186-1-Ig (same clone as 60186-1-PBS)

  Western blot of Cyclin D1 in HepG2, SW 1990 and NIH/3T3 cell lines with 60186-1-Ig at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60186-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 60186-1-Ig (same clone as 60186-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 60186-1-Ig (Cyclin D1 antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60186-1-PBS in a different storage buffer formulation.

• 

  WB analysis of A549 using 60186-1-Ig (same clone as 60186-1-PBS)

  A549 cells were subjected to SDS PAGE followed by western blot with 60186-1-Ig (Cyclin D1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 60186-1-PBS in a different storage buffer formulation.

• 

  FC experiment of SH-SY5Y using 60186-1-Ig (same clone as 60186-1-PBS)

  1X10^6 SH-SY5Y cells were stained with 0.2 ug Anti-Human Cyclin D1 (60186-1-Ig, Clone:2G3G5) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (green), and 0.2 ug Control Antibody. Cells were fixed with 90% MeOH. This data was developed using the same antibody clone with 60186-1-PBS in a different storage buffer formulation.

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Recommended dilution

Application	Dilution
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Product Information

60186-1-PBS targets Cyclin D1 in WB, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: Cyclin D1 fusion protein Ag0689
• Full Name: cyclin D1
• Calculated Molecular Weight: 295 aa, 34 kDa
• Observed Molecular Weight: 34 kDa
• GenBank Accession Number: BC000076
• Gene Symbol: CCND1
• Gene ID (NCBI): 595
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS only
• Storage Conditions: Store at -80°C.

Background Information

CCND1 (cyclin D1), also known as PRAD1 or BCL1, belongs to the highly conserved cyclin family, whose members are characterized by a dramatic periodicity in protein abundance throughout the cell cycle. CCND1 forms a complex with and functions as a regulatory subunit of CDK4 or CDK6, whose activity is required for cell cycle G1/S transition. The CCND1 gene, located on 11q13 has been reported to be overexpressed in mantle cell lymphoma (MCL) due to the chromosomal translocation. CCND1 has been shown to interact with tumor suppressor protein Rb and the expression of this gene is regulated positively by Rb. Over-expression of CCND1 is known to correlate with the early onset of cancer and risk of tumor progression and metastasis.