Western Blot Video Protocol
A simple and easy to follow Western blot video protocol.
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- Western Blot Protocol
- How To Optimize Your Western Blot
- SDS-PAGE Gel Recipes
- How To Optimize Your Results With Low MW Proteins
- Tricine Gel Recipe For Low Molecular Weight Proteins
- Choosing The Right Lysis Buffer
- Choosing The Right Western Blot Detection Method
- Western Blot Troubleshooting: Why Does The Observed Protein Molecular Weight (MW) Differ From The Calculated One?
- Western Blot Troubleshooting: High Background
- Western Blot Troubleshooting: Weak/No Signal & Other
- Western Blot ppt
- Western Blot Video Protocol
Western Blot video protocol by Proteintech Group
Western blot is a widely used analytical technique used to detect specific proteins in the given sample of tissue homogenate or extract. It uses gel electrophoresis to separate native or denatured proteins by the length of the polypeptide (denaturing conditions) or by the 3-D structure of the protein (native/ non-denaturing conditions). The proteins are then transferred to a membrane (typically nitrocellulose or PVDF), where they are probed (detected) using antibodies specific to the target protein.
The video shown is Proteintech Group's preferred method.