Standard Workflow

Labeling 0.5µg of primary antibody  

Before you begin, equilibrate all reagents to room temperature

Procedure:  

1. Combine 0.5µg of primary antibody with 1µL of FlexLinker. Add FlexBuffer to bring total volume to 8µL.

2. Mix gently and incubate for 5 minutes at room temperature in the dark.

3. Add 2µL of FlexQuencher.

4. Mix gently and incubate for 5 minutes at room temperature in the dark.

5. The antibody is now ready to be used.

Scale up proportionately to label larger quantities of primary antibody