Multiplexing

Notes on Multiplexing with the FlexAble and FlexAble 2.0 Antibody Labeling Kits

Please refer to the "Standard Workflow" section for amounts of each kit component to use per reaction. One reaction is 0.5µg of primary antibody.

Primary antibodies of different species can be combined in the same tube and labeled simultaneously using corresponding species-specific FlexLinkers. Scale up the reagent volumes appropriately based on the amount of primary antibody to be labeled.

For primary antibodies of the same species, label each antibody individually. Once the primary antibodies are labeled, combine and use in your desired application.

Antibodies labeled with the FlexAble and FlexAble 2.0 kits can be used  alongside pre-conjugated primary antibodies.