1X10^6 mouse splenocytes were surface stained with 0.5 ug CoraLite® Plus 488 Anti-Mouse CD45R (B220) (CL488-65139, Clone:RA3-6B2) or 0.5 ug CoraLite® Plus 488 Rat IgG2a Isotype Control (2A3) (CL488-65209, Clone: 2A3), and 0.5 ug CoraLite® Plus 647 Anti-Mouse CD3 (17A2) (CL647-65077, Clone: 17A2). Cells were not fixed.
Immunofluorescent analysis of mouse splenocytes using CoraLite® Plus 488-conjugated CD45R (B220) antibody (CL488-65139, Clone: RA3-6B2, green) at dilution of 1:400, and CoraLite® Plus 647-conjugated CD3 antibody (CL647-65077, Clone: 17A2, red).