VWF Recombinant antibody, PBS Only (Capture)

VWF Uni-rAb^TM Recombinant Antibody for WB, Cytometric bead array, Indirect ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, Cytometric bead array, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

240867D10

Cat no : 83854-2-PBS

Synonyms

VWF, VWFpp, VWD, von Willebrand antigen II, F8VWF, 240867D10

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Validation Data Gallery

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  WB analysis using 83854-2-RR (same clone as 83854-2-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 83854-2-RR (vwf antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 83854-2-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP00810-2

  Cytometric bead array standard curve of MP00810-2, VWF Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 83854-2-PBS. Detection antibody: 83854-1-PBS. Standard: Ag25578. Range: 0.313-40 ng/mL.

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  Affinity and Kinetic Characterization of 83854-2-RR

  Biolayer interferometry (BLl) kinetic assays of 83854-2-RR against Human VWF were performed. The affinity constant is 0.965 nM.

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Product Information

83854-2-PBS targets VWF as part of a matched antibody pair:

MP00810-2: 83854-2-PBS capture and 83854-1-PBS detection (validated in Cytometric bead array)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: VWF fusion protein Ag25578
• Full Name: von Willebrand factor
• Observed Molecular Weight: 309-320 kDa
• Gene Symbol: VWF
• Gene ID (NCBI): 7450
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Von Willebrand factor (VWF) is a large multimeric glycoprotein found in blood plasma involved in hemostasis following vascular injury. Due to the multimeric nature of VWF, it can range in size from 500 to 20,000 kDa due to the differences in the number of subunits comprising the protein. Each subunit is approximately 250 kDa (PMID: 9759493). The biosynthesis of VWF in vivo is limited to endothelial cells (PMID: 4209883) and megakaryocytes (PMID: 2413071). VWF synthesized in endothelial cells is either released directly into the plasma via 27186a secretory pathway, or tubulized and stored in organelles unique to this cell type called Weibel-Palade bodies (PMID: 16459301). Whereas VWF synthesized in megakaryocytes is stored in the alpha granules of platelets (PMID: 2046403). The primary function of VWF is as an adhesive plasma glycoprotein, particularly factor VIII; an essential blood-clotting protein (PMID: 6982084). VWF is also important in platelet adhesion to wound sites by binding specifically to type I and type III collagen (PMID: 11098050), with larger VWF multimers being most effective (PMID: 24448155).