VWF Recombinant antibody

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  WB analysis using 83854-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83854-2-RR (vwf antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human placenta using 83854-2-RR

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 83854-2-RR (VWF antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary cancer using 83854-2-RR

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 83854-2-RR (VWF antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary cancer using 83854-2-RR

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 83854-2-RR (VWF antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 83854-2-RR

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 83854-2-RR (VWF antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  Affinity and Kinetic Characterization of 83854-2-RR

  Biolayer interferometry (BLl) kinetic assays of 83854-2-RR against Human VWF were performed. The affinity constant is 0.965 nM.

VWF Uni-rAb^TM Recombinant Antibody for WB, IHC, ELISA

Cat No. 83854-2-RR

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, IHC, ELISA

Synonyms

VWF, VWFpp, VWD, von Willebrand antigen II, F8VWF, 240867D10

Formulation:  PBS and Azide

PBS and Azide

PBS Only

Conjugate:  Unconjugated

Unconjugated

Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

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Tested Applications

• Positive WB detected in: human placenta tissue, rat spleen tissue
• Positive IHC detected in: human tonsillitis tissue, human ovary cancer tissue, human placenta tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

83854-2-RR targets VWF in WB, IHC, ELISA applications and shows reactivity with human, rat samples.

• Tested Reactivity: human, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: VWF fusion protein Ag25578
• Full Name: von Willebrand factor
• Observed Molecular Weight: 309-320 kDa
• Gene Symbol: VWF
• Gene ID (NCBI): 7450
• RRID: AB_3671439
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P04275
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Von Willebrand factor (VWF) is a large multimeric glycoprotein found in blood plasma involved in hemostasis following vascular injury. Due to the multimeric nature of VWF, it can range in size from 500 to 20,000 kDa due to the differences in the number of subunits comprising the protein. Each subunit is approximately 250 kDa (PMID: 9759493). The biosynthesis of VWF in vivo is limited to endothelial cells (PMID: 4209883) and megakaryocytes (PMID: 2413071). VWF synthesized in endothelial cells is either released directly into the plasma via 27186a secretory pathway, or tubulized and stored in organelles unique to this cell type called Weibel-Palade bodies (PMID: 16459301). Whereas VWF synthesized in megakaryocytes is stored in the alpha granules of platelets (PMID: 2046403). The primary function of VWF is as an adhesive plasma glycoprotein, particularly factor VIII; an essential blood-clotting protein (PMID: 6982084). VWF is also important in platelet adhesion to wound sites by binding specifically to type I and type III collagen (PMID: 11098050), with larger VWF multimers being most effective (PMID: 24448155).