Gamma Tubulin Monoclonal antibody
Gamma Tubulin Monoclonal Antibody for IF, IHC, WB, ELISA
Host / Isotype
Mouse / IgG2a
Reactivity
human, Canine, mouse, rat
Applications
WB, IHC, IF, ELISA
Conjugate
Unconjugated
CloneNo.
3F9H8
Cat no : 66320-1-Ig
Synonyms
"Gamma Tubulin Antibodies" Comparison
View side-by-side comparison of Gamma Tubulin antibodies from other vendors to find the one that best suits your research needs.
Tested Applications
Positive WB detected in | NCCIT cells, HepG2 cells, EC109 cells, K-562 cells, HSCT6 cells, NIH3T3 cells |
Positive IHC detected in | human breast cancer tissue, human prostate cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | MDCK cells, HeLa cells, A549 cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:2000-1:12000 |
Immunohistochemistry (IHC) | IHC : 1:200-1:1000 |
Immunofluorescence (IF) | IF : 1:50-1:500 |
Sample-dependent, check data in validation data gallery |
Published Applications
WB | See 3 publications below |
IF | See 6 publications below |
Product Information
66320-1-Ig targets Gamma Tubulin in WB, IHC, IF, ELISA applications and shows reactivity with human, Canine, mouse, rat samples.
Tested Reactivity | human, Canine, mouse, rat |
Cited Reactivity | human, mouse |
Host / Isotype | Mouse / IgG2a |
Class | Monoclonal |
Type | Antibody |
Immunogen | Gamma Tubulin fusion protein Ag23913 |
Full Name | tubulin, gamma 1 |
Calculated molecular weight | 51 kDa |
Observed molecular weight | 48-55 kDa |
GenBank accession number | BC000619 |
Gene symbol | TUBG1 |
Gene ID (NCBI) | 7283 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
Gamma tubulin is a member of tubulin superfamily and is a key component required for microtubule nucleation and stabilization. It is concentrated at the pericentriolar material of centrosomes in interphase cells, predominantly on spindle poles in mitotic cells, while found in midbodies during cytokinesis. Overexpression of gamma tubulin has been found in various cancers including breast cancer and gliomas. This antibody can well label the centrosome structure.
Protocols
Product Specific Protocols | |
---|---|
WB protocol for Gamma Tubulin antibody 66320-1-Ig | Download protocol |
IHC protocol for Gamma Tubulin antibody 66320-1-Ig | Download protocol |
IF protocol for Gamma Tubulin antibody 66320-1-Ig | Download protocol |
Standard Protocols | |
---|---|
Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Oncotarget Prohibitin, relocated to the front ends, can control the migration directionality of colorectal cancer cells. | ||
J Comp Neurol Distribution of prototypical primary cilia markers in subtypes of retinal ganglion cells. | ||
J Nat Prod The Phytochemical Scoulerine Inhibits Aurora Kinase Activity to Induce Mitotic and Cytokinetic Defects. | ||
Biomed Pharmacother The phytochemical, corynoline, diminishes Aurora kinase B activity to induce mitotic defect and polyploidy. | ||
Cancers (Basel) Polycaprolactone Electrospun Scaffolds Produce an Enrichment of Lung Cancer Stem Cells in Sensitive and Resistant EGFRm Lung Adenocarcinoma. | ||
Elife Respiratory syncytial virus co-opts host mitochondrial function to favour infectious virus production. |