Gamma Tubulin Monoclonal antibody

• Validation Data Gallery
• Proteintech vs. Other Vendors

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  WB analysis using 66320-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66320-1-Ig (gamma tubulin antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 66320-1-Ig

  K-562 cells were subjected to SDS PAGE followed by western blot with 66320-1-Ig (gamma tubulin antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 66320-1-Ig

  HepG2 cells were subjected to SDS PAGE followed by western blot with 66320-1-Ig (gamma tubulin antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of EC109 using 66320-1-Ig

  EC109 cells were subjected to SDS PAGE followed by western blot with 66320-1-Ig (gamma tubulin antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 66320-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66320-1-Ig (gamma tubulin antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 66320-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66320-1-Ig (gamma tubulin antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human prostate cancer using 66320-1-Ig

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66320-1-Ig (gamma tubulin antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human prostate cancer using 66320-1-Ig

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66320-1-Ig (gamma tubulin antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of MDCK using 66320-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed MDCK cells using 66320-1-Ig (gamma tubulin antibody) at dilution of 1:200 and CoraLite488-Conjugated Goat Anti-Mouse IgG(H+L).

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  IF Staining of MDCK using 66320-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed MDCK cells using 66320-1-Ig (gamma tubulin antibody) at dilution of 1:200 and CoraLite488-Conjugated Goat Anti-Mouse IgG(H+L).

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  IF Staining of A549 using 66320-1-Ig

  Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using Gamma Tubulin antibody (66320-1-Ig, Clone: 3F9H8 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), Alpha Tubulin antibody (11224-1-AP, red).

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  IF Staining of HeLa using 66320-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 66320-1-Ig (gamma tubulin antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated Goat Anti-Mouse IgG(H+L).

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  FC experiment of HeLa using 66320-1-Ig

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human Gamma Tubulin (66320-1-Ig, Clone:3F9H8) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Gamma Tubulin Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Cat No. 66320-1-Ig

Clone No.3F9H8

Publications(30)

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat, canine

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Synonyms

TUBG1, TUBG, GCP-1, Gamma-tubulin complex component 1, Gamma-1-tubulin

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Tested Applications

• Positive WB detected in: NCCIT cells, HepG2 cells, EC109 cells, K-562 cells, HSCT6 cells, NIH3T3 cells
• Positive IHC detected in: human breast cancer tissue, human prostate cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: MDCK cells, HeLa cells, A549 cells
• Positive FC (Intra) detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:12000
• Immunohistochemistry (IHC): IHC : 1:200-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 13 publications below
• IF: See 21 publications below

Product Information

66320-1-Ig targets Gamma Tubulin in WB, IHC, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat, canine samples.

• Tested Reactivity: human, mouse, rat, canine
• Cited Reactivity: human, mouse
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: Gamma Tubulin fusion protein Ag23913
• Full Name: tubulin, gamma 1
• Calculated Molecular Weight: 51 kDa
• Observed Molecular Weight: 48-55 kDa
• GenBank Accession Number: BC000619
• Gene Symbol: Gamma Tubulin
• Gene ID (NCBI): 7283
• RRID: AB_2857350
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P23258
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Gamma tubulin is a member of tubulin superfamily and is a key component required for microtubule nucleation and stabilization. It is concentrated at the pericentriolar material of centrosomes in interphase cells, predominantly on spindle poles in mitotic cells, while found in midbodies during cytokinesis. Overexpression of gamma tubulin has been found in various cancers including breast cancer and gliomas. This antibody can well label the centrosome structure.

Publications

Species	Application	Title
human	WB	Nature; Structure of LRRK2 in Parkinson's disease and model for microtubule interaction.; Authors - C K Deniston
	IF	EMBO Mol Med; Mutations in GRK2 cause Jeune syndrome by impairing Hedgehog and canonical Wnt signaling.; Authors - Michaela Bosakova
mouse	WB	J Cell Biol; Time-resolved proteomics profiling of the ciliary Hedgehog response.; Authors - Elena A May
	IF	Cell Rep; MYO10 regulates genome stability and cancer inflammation through mediating mitosis; Authors - Franklin Mayca Pozo
human	IF	Elife; Respiratory syncytial virus co-opts host mitochondrial function to favour infectious virus production.; Authors - MengJie Hu
human	WB,IF	J Nanobiotechnology; GRP75-driven, cell-cycle-dependent macropinocytosis of Tat/pDNA-Ca2+ nanoparticles underlies distinct gene therapy effect in ovarian cancer.; Authors - Linjia Su