Gamma Tubulin Monoclonal antibody

Gamma Tubulin Monoclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, Canine, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

CloneNo.

3F9H8

Cat no : 66320-1-Ig

Synonyms

Gamma 1 tubulin, Gamma Tubulin, GCP 1, TUBG, TUBG1, TUBGCP1, Tubulin, Tubulin gamma 1 chain, tubulin, gamma 1, tubulin-gamma


"Gamma Tubulin Antibodies" Comparison

View side-by-side comparison of Gamma Tubulin antibodies from other vendors to find the one that best suits your research needs.
Applications:
  • WB analysis using 66320-1-Ig

    WB analysis using 66320-1-Ig

    Various lysates were subjected to SDS PAGE followed by western blot with 66320-1-Ig (gamma tubulin antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

  • WB analysis of HepG2 using 66320-1-Ig

    WB analysis of HepG2 using 66320-1-Ig

    HepG2 cells were subjected to SDS PAGE followed by western blot with 66320-1-Ig (gamma tubulin antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis of EC109 using 66320-1-Ig

    WB analysis of EC109 using 66320-1-Ig

    EC109 cells were subjected to SDS PAGE followed by western blot with 66320-1-Ig (gamma tubulin antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • WB analysis of K-562 using 66320-1-Ig

    WB analysis of K-562 using 66320-1-Ig

    K-562 cells were subjected to SDS PAGE followed by western blot with 66320-1-Ig (gamma tubulin antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  • IHC staining of human breast cancer using 66320-1-Ig

    IHC staining of human breast cancer using 66320-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66320-1-Ig (gamma tubulin antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human breast cancer using 66320-1-Ig

    IHC staining of human breast cancer using 66320-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66320-1-Ig (gamma tubulin antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human prostate cancer using 66320-1-Ig

    IHC staining of human prostate cancer using 66320-1-Ig

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66320-1-Ig (gamma tubulin antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IHC staining of human prostate cancer using 66320-1-Ig

    IHC staining of human prostate cancer using 66320-1-Ig

    Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66320-1-Ig (gamma tubulin antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  • IF Staining of MDCK using 66320-1-Ig

    IF Staining of MDCK using 66320-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed MDCK cells using 66320-1-Ig (gamma tubulin antibody) at dilution of 1:200 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • IF Staining of MDCK using 66320-1-Ig

    IF Staining of MDCK using 66320-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed MDCK cells using 66320-1-Ig (gamma tubulin antibody) at dilution of 1:200 and CoraLite488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

  • IF Staining of A549 using 66320-1-Ig

    IF Staining of A549 using 66320-1-Ig

    Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using Gamma Tubulin antibody (66320-1-Ig, Clone: 3F9H8 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), Alpha Tubulin antibody (11224-1-AP, red).

  • IF Staining of HeLa using 66320-1-Ig

    IF Staining of HeLa using 66320-1-Ig

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 66320-1-Ig (gamma tubulin antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).


Tested Applications

Positive WB detected inNCCIT cells, HepG2 cells, EC109 cells, K-562 cells, HSCT6 cells, NIH3T3 cells
Positive IHC detected inhuman breast cancer tissue, human prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inMDCK cells, HeLa cells, A549 cells
Planning an IF experiment? We recommend our CoraLite®594 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:12000
Immunohistochemistry (IHC)IHC : 1:200-1:1000
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

66320-1-Ig targets Gamma Tubulin in WB, IHC, IF, ELISA applications and shows reactivity with human, Canine, mouse, rat samples.

Tested Reactivity human, Canine, mouse, rat
Cited Reactivityhuman, mouse
Host / Isotype Mouse / IgG2a
Class Monoclonal
Type Antibody
Immunogen Gamma Tubulin fusion protein Ag23913
Full Name tubulin, gamma 1
Calculated molecular weight 51 kDa
Observed molecular weight 48-55 kDa
GenBank accession numberBC000619
Gene symbol TUBG1
Gene ID (NCBI) 7283
Conjugate Unconjugated
Form Liquid
Purification Method Protein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Gamma tubulin is a member of tubulin superfamily and is a key component required for microtubule nucleation and stabilization. It is concentrated at the pericentriolar material of centrosomes in interphase cells, predominantly on spindle poles in mitotic cells, while found in midbodies during cytokinesis. Overexpression of gamma tubulin has been found in various cancers including breast cancer and gliomas. This antibody can well label the centrosome structure.

Protocols

Product Specific Protocols
WB protocol for Gamma Tubulin antibody 66320-1-IgDownload protocol
IHC protocol for Gamma Tubulin antibody 66320-1-IgDownload protocol
IF protocol for Gamma Tubulin antibody 66320-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Oncotarget

Prohibitin, relocated to the front ends, can control the migration directionality of colorectal cancer cells.

Authors - Li-Li Ma
mouseIF

J Comp Neurol

Distribution of prototypical primary cilia markers in subtypes of retinal ganglion cells.

Authors - Tia J Kowal
humanIF

J Nat Prod

The Phytochemical Scoulerine Inhibits Aurora Kinase Activity to Induce Mitotic and Cytokinetic Defects.

Authors - Jinhua Li
humanIF

Biomed Pharmacother

The phytochemical, corynoline, diminishes Aurora kinase B activity to induce mitotic defect and polyploidy.

Authors - Ziqi Yan
humanWB

Cancers (Basel)

Polycaprolactone Electrospun Scaffolds Produce an Enrichment of Lung Cancer Stem Cells in Sensitive and Resistant EGFRm Lung Adenocarcinoma.

Authors - Emma Polonio-Alcalá
humanIF

Elife

Respiratory syncytial virus co-opts host mitochondrial function to favour infectious virus production.

Authors - MengJie Hu