Gamma Tubulin Monoclonal antibody, PBS Only

Gamma Tubulin Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse, rat, canine

Applications

WB, IHC, IF/ICC, FC (Intra), Indirect ELISA

Conjugate

Unconjugated

CloneNo.

3F9H8

Cat no : 66320-1-PBS

Synonyms

TUBG1, TUBG, GCP-1, Gamma-tubulin complex component 1, Gamma-1-tubulin

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Validation Data Gallery

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  WB analysis using 66320-1-Ig (same clone as 66320-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66320-1-Ig (gamma tubulin antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66320-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 66320-1-Ig (same clone as 66320-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 66320-1-Ig (gamma tubulin antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66320-1-PBS in a different storage buffer formulation.

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  WB analysis of EC109 using 66320-1-Ig (same clone as 66320-1-PBS)

  EC109 cells were subjected to SDS PAGE followed by western blot with 66320-1-Ig (gamma tubulin antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66320-1-PBS in a different storage buffer formulation.

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  WB analysis of K-562 using 66320-1-Ig (same clone as 66320-1-PBS)

  K-562 cells were subjected to SDS PAGE followed by western blot with 66320-1-Ig (gamma tubulin antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66320-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 66320-1-Ig (same clone as 66320-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66320-1-Ig (gamma tubulin antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66320-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 66320-1-Ig (same clone as 66320-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 66320-1-Ig (gamma tubulin antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66320-1-PBS in a different storage buffer formulation.

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  IHC staining of human prostate cancer using 66320-1-Ig (same clone as 66320-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66320-1-Ig (gamma tubulin antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66320-1-PBS in a different storage buffer formulation.

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  IHC staining of human prostate cancer using 66320-1-Ig (same clone as 66320-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 66320-1-Ig (gamma tubulin antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66320-1-PBS in a different storage buffer formulation.

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  IF Staining of MDCK using 66320-1-Ig (same clone as 66320-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed MDCK cells using 66320-1-Ig (gamma tubulin antibody) at dilution of 1:200 and CoraLite488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66320-1-PBS in a different storage buffer formulation.

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  IF Staining of MDCK using 66320-1-Ig (same clone as 66320-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed MDCK cells using 66320-1-Ig (gamma tubulin antibody) at dilution of 1:200 and CoraLite488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66320-1-PBS in a different storage buffer formulation.

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  IF Staining of A549 using 66320-1-Ig (same clone as 66320-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed A549 cells using Gamma Tubulin antibody (66320-1-Ig, Clone: 3F9H8 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L), Alpha Tubulin antibody (11224-1-AP, red). This data was developed using the same antibody clone with 66320-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 66320-1-Ig (same clone as 66320-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 66320-1-Ig (gamma tubulin antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 66320-1-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 66320-1-Ig (same clone as 66320-1-PBS)

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human Gamma Tubulin (66320-1-Ig, Clone:3F9H8) and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Mouse IgG2a Isotype Control (C1.18.4) (65208-1-Ig, Clone: C1.18.4) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C). This data was developed using the same antibody clone with 66320-1-PBS in a different storage buffer formulation.

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Product Information

66320-1-PBS targets Gamma Tubulin in WB, IHC, IF/ICC, FC (Intra), Indirect ELISA applications and shows reactivity with human, mouse, rat, canine samples.

• Tested Reactivity: human, mouse, rat, canine
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: Gamma Tubulin fusion protein Ag23913
• Full Name: tubulin, gamma 1
• Calculated Molecular Weight: 51 kDa
• Observed Molecular Weight: 48-55 kDa
• GenBank Accession Number: BC000619
• Gene Symbol: Gamma Tubulin
• Gene ID (NCBI): 7283
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Gamma tubulin is a member of tubulin superfamily and is a key component required for microtubule nucleation and stabilization. It is concentrated at the pericentriolar material of centrosomes in interphase cells, predominantly on spindle poles in mitotic cells, while found in midbodies during cytokinesis. Overexpression of gamma tubulin has been found in various cancers including breast cancer and gliomas. This antibody can well label the centrosome structure.