CoraLite®594-conjugated smooth muscle actin specific Recombinant antibody

• Featured Product
• KD/KO Validated

smooth muscle actin specific Uni-rAb^TM Recombinant Antibody for IF/ICC, IF-P, FC (Intra)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat, pig, chicken

Applications

WB, IF/ICC, IF-P, FC (Intra)

Conjugate

CoraLite®594 Fluorescent Dye

Publications

1

CloneNo.

5H7

Cat no : CL594-80008

Synonyms

ACTA2, Alpha SMA, a-SMA, smooth muscle actin, Actin, aortic smooth muscle, intermediate form

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Validation Data Gallery

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  IF Staining of mouse heart using CL594-80008

  Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using CoraLite®594 smooth muscle actin specific antibody (CL594-80008, Clone: 5H7 ) at dilution of 1:200.

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  IF Staining of H9C2 using CL594-80008

  Immunofluorescent analysis of (-20°C Ethanol) fixed H9C2 cells using CoraLite®594 smooth muscle actin specific antibody (CL594-80008, Clone: 5H7 ) at dilution of 1:200.

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  FC experiment of C2C12 using CL594-80008

  1X10^6 C2C12 cells were intracellularly stained with 0.4 ug CoraLite®594 Anti-Human smooth muscle actin specific (CL594-80008, Clone:5H7) (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Tested Applications

• Positive IF-P detected in: mouse heart tissue
• Positive IF/ICC detected in: H9C2 cells
• Positive FC (Intra) detected in: C2C12 cells

Recommended dilution

• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

CL594-80008 targets smooth muscle actin specific in WB, IF/ICC, IF-P, FC (Intra) applications and shows reactivity with human, mouse, rat, pig, chicken samples.

• Tested Reactivity: human, mouse, rat, pig, chicken
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Peptide
• Full Name: actin, alpha 2, smooth muscle, aorta
• Calculated Molecular Weight: 42 kDa
• Observed Molecular Weight: 42 kDa
• GenBank Accession Number: NM_001613
• Gene Symbol: Alpha smooth muscle actin
• Gene ID (NCBI): 59
• Conjugate: CoraLite®594 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 588 nm / 604 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

ACTA2 (also known as α-smooth muscle actin or α-SMA) belongs to the actin family. Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. ACTA2 is primarily expressed in vascular smooth muscle and anti-ACTA2 is commonly used to marker smooth muscle cells.

Publications

Species	Application	Title
human	WB	Toxicol Appl Pharmacol; Mechanistic role for mTORC1 signaling in profibrotic toxicity of low-dose cadmium; Authors - Choon-Myung Lee