Tested Applications
| Positive IF-P detected in | mouse heart tissue |
| Positive IF-Fro detected in | mouse small intestine tissue |
| Positive IF/ICC detected in | H9C2 cells |
| Positive FC (Intra) detected in | C2C12 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| Immunofluorescence (IF)-FRO | IF-FRO : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
CL594-80008 targets Alpha smooth muscle actin specific in WB, IF/ICC, IF-P, IF-Fro, FC (Intra) applications and shows reactivity with human, mouse, rat, pig, chicken samples.
| Tested Reactivity | human, mouse, rat, pig, chicken |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Recombinant |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | actin, alpha 2, smooth muscle, aorta |
| Calculated Molecular Weight | 42 kDa |
| Observed Molecular Weight | 42 kDa |
| GenBank Accession Number | NM_001613 |
| Gene Symbol | Alpha smooth muscle actin |
| Gene ID (NCBI) | 59 |
| RRID | AB_2934798 |
| Conjugate | CoraLite®594 Fluorescent Dye |
| Excitation/Emission Maxima Wavelengths | 588 nm / 604 nm |
| Excitation Laser | Yellow-Green Laser (561 nm) |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P62736 |
| Storage Buffer | PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3. |
| Storage Conditions | Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. |
Background Information
ACTA2 (also known as α-smooth muscle actin or α-SMA) belongs to the actin family. Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. ACTA2 is primarily expressed in vascular smooth muscle and anti-ACTA2 is commonly used to marker smooth muscle cells.
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for CL594 Alpha smooth muscle actin specific antibody CL594-80008 | Download protocol |
| IF protocol for CL594 Alpha smooth muscle actin specific antibody CL594-80008 | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
CL594-80008 is the CoraLite®594-conjugated α-smooth muscle actin (α-SMA) specific recombinant monoclonal antibody (clone 5H7). It has 2 total citations. The citations appear in Toxicological Sciences (Toxicol Appl Pharmacol) and Chemistry & Biodiversity (Chem Biodivers). The associated research fields include mTORC1 signaling in profibrotic toxicity of low-dose cadmium, and network pharmacology with experimental validation of Ficus tikoua for ameliorating liver fibrosis. Both studies applied the antibody via Western blot (WB) in human and/or mouse samples.
| Species | Application | Title |
|---|---|---|
Toxicol Appl Pharmacol Mechanistic role for mTORC1 signaling in profibrotic toxicity of low-dose cadmium | ||
Chem Biodivers UPLC-MS/MS-Network Pharmacology and Experimental Validation Reveal Active Components of Ficus tikoua for Ameliorating Liver Fibrosis. |
