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smooth muscle actin specific Recombinant antibody

smooth muscle actin specific Recombinant Antibody for FC, IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG


Human, mouse, rat, chicken, pig, zebrafish and More (1)







Cat no : 80008-1-RR


a SMA, AAT6, ACTA2, ACTA2 Smooth muscle Actin, Actin, aortic smooth muscle, ACTSA, ACTVS, Alpha actin 2, Alpha SMA, a-SMA, SMA, smooth muscle actin, smooth muscle actin specific, smooth muscle actin-specific

Tested Applications

Positive WB detected inmouse colon tissue, pig vein tissue, chicken crop tissue, zebrafish tissue, A549 cells, pig stomach, pig colon, rat stomach, chicken small intestine tissue, rat colon tissue
Positive IHC detected inhuman liver tissue, human heart tissue, human appendicitis tissue, mouse colon tissue, rat colon tissue, human colon tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inmouse heart tissue, mouse embryo tissue
Positive FC detected inC2C12 cells

Recommended dilution

Western Blot (WB)WB : 1:20000-1:100000
Immunohistochemistry (IHC)IHC : 1:2500-1:10000
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.40 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

80008-1-RR targets smooth muscle actin specific in WB, IHC, IF, FC, ELISA applications and shows reactivity with Human, mouse, rat, chicken, pig, zebrafish samples.

Tested Reactivity Human, mouse, rat, chicken, pig, zebrafish
Cited Reactivityhuman, mouse, rat
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide
Full Name actin, alpha 2, smooth muscle, aorta
Calculated Molecular Weight 42 kDa
Observed Molecular Weight42 kDa
GenBank Accession NumberNM_001613
Gene Symbol ACTA2
Gene ID (NCBI) 59
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

ACTA2 (also known as α-smooth muscle actin or α-SMA) belongs to the actin family. Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. ACTA2 is primarily expressed in vascular smooth muscle and anti-ACTA2 is commonly used to marker smooth muscle cells.


Product Specific Protocols
WB protocol for smooth muscle actin specific antibody 80008-1-RRDownload protocol
IHC protocol for smooth muscle actin specific antibody 80008-1-RRDownload protocol
IF protocol for smooth muscle actin specific antibody 80008-1-RRDownload protocol
FC protocol for smooth muscle actin specific antibody 80008-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols




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