CoraLite® Plus 488-conjugated smooth muscle actin specific Polyclonal antibody

smooth muscle actin specific Polyclonal Antibody for IF-P

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF-P

Conjugate

CoraLite® Plus 488 Fluorescent Dye

Cat no : CL488-55135

Synonyms

ACTA2, Alpha SMA, a-SMA, smooth muscle actin, Alpha actin 2

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Validation Data Gallery

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  IF Staining of mouse heart using CL488-55135

  Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using CoraLite® Plus 488 smooth muscle actin-specific antibody (CL488-55135) at dilution of 1:200.

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  IF Staining of mouse spleen using CL488-55135

  Immunofluorescent analysis of (4% PFA) fixed mouse spleen tissue using CoraLite® Plus 488 smooth muscle actin specific antibody (CL488-55135) at dilution of 1:200, F4/80 antibody (28463-1-AP, Magenta).

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Tested Applications

• Positive IF-P detected in: mouse heart tissue, mouse spleen tissue

This antibody is recommended for use in IHC.

Recommended dilution

• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-55135 targets smooth muscle actin specific in IF-P applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: actin, alpha 2, smooth muscle, aorta
• Calculated Molecular Weight: 42 kDa
• Observed Molecular Weight: 42 kDa
• GenBank Accession Number: NM_001613
• Gene Symbol: Alpha smooth muscle actin
• Gene ID (NCBI): 59
• RRID: AB_2919221
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

ACTA2, also named as a-SMA,AAT6;ACTSA belongs to the actin family. Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. Defects in ACTA2 are the cause of aortic aneurysm familial thoracic type 6 (AAT6). The immunogen of this antibody is a specific domain of ACTA2. This antibody stains the aorta only.