HumanKine® recombinant human Noggin protein- GMP grade

• HEK293 expressed
• Endotoxin-free
• Animal-component free

EC50

1.5-15 ng/mL

Species Reactivity

human,mouse

Purity

>95%

Cat no : HZ-1118-GMP

Synonyms

NOG, noggin, SYM1, SYNS1

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Validation Data Gallery

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  Purity of recombinant human Noggin was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

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  Recombinant human Noggin (HZ-1118-GMP) inhibits dose-dependent induction of alkaline phosphatase production by BMP-4 in the ATDC-5 mouse chondrogenic cell line. Alkaline phosphatase production was assessed using pNPP as a chromogenic substrate. ATDC-5 cells were treated with increasing concentrations of recombinant human Noggin and 40 ng/mL of BMP-4 (HZ-1045) for 72 hrs hours before lysis and addition of pNPP. The EC50 was determined using a 4-parameter non-linear regression model. Activity determination was conducted in triplicate on a validated bioassay. The EC50 values range from 3-15 ng/mL.

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Technical Specifications

• GeneID: 9241
• Species: Human
• Expression: HEK293
• EC50: 1.5-15 ng/mL
• Purity: >95%
• Endotoxin: <0.1 EU/μg
• Accession Number: Q13253
• Molecular Mass: 28 kDa reduced, 50 to 95 kDa non-reduced, homodimer, glycosylated
• Formulation: 1x PBS, See Certificate of Analysis for details
• Species Reactivity: human,mouse

Stability and Reconstitution

Stability and Storage	Product Form	Temperature Conditions	Storage Time (From Date of Receipt)
	Lyophilized	-20°C to -80°C	Until Expiry Date
	Lyophilized	Room Temperature	2 weeks
	Reconstituted as per CofA	-20°C to -80°C	6 months
	Reconstituted as per CofA	4°C	1 week
	Avoid repeated freeze-thaw cycles.

Reconstitution

Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein 0.2 mg/mL in sterile 1xPBS pH 7.4 containing 0.1% endotoxin-free recombinant human serum albumin (HSA). Gently swirl or tap vial to mix.

GMP Quality Policies

HumanKine® GMP (Good Manufacturing Practice) recombinant proteins are manufactured and validated in accordance with ISO 13485 quality management system and is compliant with GMP.
Our GMP recombinant proteins are animal component free (ACF), xeno free (XF) and tag free (TF). Read more about these policies here.

Whitepapers

HumanKine® Noggin Human Cell Expressed Glycosylated Disulfide-linked Homodimer

Background

Noggin is an inhibitor of bone morphogenetic proteins (BMP) signaling which is required for growth and patterning of the neural tube and somite. It is essential for cartilage morphogenesis and joint formation. Noggin inhibits chondrocyte differentiation through its interaction with GDF5 and GDF6 (PMID: 29497255; 29167826; 29094227; 28842965).

Synonyms

NOG, noggin, SYM1, SYNS1