• HEK293 expressed
  • Endotoxin-free
  • Animal-component free

HumanKine® recombinant human IL-3 protein

Activity(Primary)

0.4-2.0 ng/mL

Specific Activity

minimally 5.0 x 105 IU/mg

Species Reactivity

human

Purity

>95%

Cat no : HZ-1074

Synonyms

Hematopoietic growth factor, IL 3, IL3, IL-3, Interleukin 3, Mast cell growth factor, MCGF, MULTI CSF, P cell stimulating factor



Technical Specifications

GeneID 3562
Species Human
Expression HEK293
Activity(Primary) 0.4-2.0 ng/mL
Specific Activityminimally 5.0 x 105 IU/mg
Purity >95%
Endotoxin<1 EU/µg
Accession NumberP08700
Molecular Mass 17 to 50 kDa reduced and non-reduced, monomer, glycosylated
Formulation 1xPBS, See Certificate of Analysis for details
Species Reactivity human

Stability and Reconstitution

Stability and StorageProduct FormTemperature ConditionsStorage Time
(From Date of Receipt)
Lyophilized-20°C to -80°CUntil Expiry Date
LyophilizedRoom Temperature2 weeks
Reconstituted as per CofA-20°C to -80°C6 months
Reconstituted as per CofA4°C1 week
Avoid repeated freeze-thaw cycles.
Reconstitution Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein to 0.2 mg/mL in sterile 1xPBS pH 7.4. Gently swirl or tap vial to mix.

Background

Interleukin-3 (IL-3) is a multilineage hematopoietic growth factor that promotes the proliferation, differentiation and survival of early multilineage hematopoietic progenitors. In particular, this cytokine plays a key role in stimulating the proliferation and survival of myeloid precursors. It is involved in a variety of cell activities such as cell growth, differentiation and apoptosis. This cytokine has been shown to also possess neurotrophic activity, and it may be associated with neurologic disorders (PMID: 24103757;2698876;2809196)

Synonyms

Hematopoietic growth factor, IL 3, IL3, IL-3, Interleukin 3, Mast cell growth factor, MCGF, MULTI CSF, P cell stimulating factor

Publications

SpeciesTitle
human

Blood

LIN28B-mediated expression of fetal hemoglobin and production of fetal-like erythrocytes from adult human erythroblasts ex vivo.

Authors - Y TerryLee
human

J Exp Med

Co-inhibition of NF-κB and JNK is synergistic in TNF-expressing human AML.

Authors - AndrewVolk
human

Mol Cancer Ther

IMGN779, a Novel CD33-Targeting Antibody-Drug Conjugate with DNA Alkylating Activity, Exhibits Potent Antitumor Activity in Models of AML.

Authors - YelenaKovtun
human

Oncotarget

Sensitizing leukemia stem cells to NF-κB inhibitor treatment in vivo by inactivation of both TNF and IL-1 signaling.

Authors - JingLi
human

Biochem Pharmacol

The new mitochondrial uncoupler BAM15 induces ROS production for treatment of acute myeloid leukemia.

Authors - Zhen Xing Gao
human

J Tissue Eng Regen Med

Iron dose-dependent differentiation and enucleation of human erythroblasts in serum-free medium.

Authors - ColleenByrnes