HumanKine® recombinant human IFN gamma protein

• HEK293 expressed
• Endotoxin-free
• Animal-component free

Activity(Primary)

0.02-0.14 ng/mL EC50

Specific Activity

>1.00 x 10⁷ IU/mg

Species Reactivity

human,mouse

Purity

>95%

Publications

17

Cat no : HZ-1301

Synonyms

IFG, IFI, IFN gamma, IFNG, Immune interferon, Interferon gamma, interferon, gamma

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Validation Data Gallery

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  Purity of recombinant human IFN gamma was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue

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  Recombinant human IFN gamma (HZ-1301) dose-dependently inhibits proliferation of the HT-29 human colorectal adenocarcinoma cell line. Cell number was quantitatively assessed by PrestoBlue® cell viability reagent. HT-29 cells were treated with increasing concentrations of recombinant IFN gamma for 72 hours. The EC50 was determined using a 4-parameter non-linear regression model.The EC50 range is 0.02-0.14 ng/mL.

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  Proteintech IFN gamma (HZ-1301) demonstrates a 3-fold lower EC50 and equivalent dose-dependent inhibition of proliferation to a leading competitor. Recombinant human IFN gamma dose-dependently inhibits proliferation of the HT-29 human colorectal adenocarcinoma cell line. Cell number was quantitatively assessed by PrestoBlue® cell viability reagent. HT-29 cells were treated with increasing concentrations of recombinant IFN gamma for 72 hours. The EC50 was determined using a 4-parameter non-linear regression model.The EC50 range is 0.02-0.14 ng/mL.

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Technical Specifications

• GeneID: 3458
• Species: Human
• Expression: HEK293
• Activity(Primary): 0.02-0.14 ng/mL EC50
• Specific Activity: >1.00 x 10^7 IU/mg
• Purity: >95%
• Endotoxin: <1 EU/μg
• Accession Number: P01579
• Molecular Mass: 29-32 kDa
• Formulation: 1x PBS, See Certificate of Analysis for details
• Species Reactivity: human,mouse

Stability and Reconstitution

Stability and Storage	Product Form	Temperature Conditions	Storage Time (From Date of Receipt)
	Lyophilized	-20°C to -80°C	Until Expiry Date
	Lyophilized	Room Temperature	2 weeks
	Reconstituted as per CofA	-20°C to -80°C	6 months
	Reconstituted as per CofA	4°C	1 week
	Avoid repeated freeze-thaw cycles.

Reconstitution

Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein in sterile 1x PBS pH 7.4 containing 0.1% endotoxin-free recombinant human albumin (HSA).

Background

Interferon gamma (IFN γ), is a type II interferon that provides immunity against bacterial, viral and protozoan infections. In its active form, IFN γ is a glycosylated, non-covalently linked homodimer of 29-32 kDa subunits. It is produced by a number of immune cell types including natural killer cells, natural killer T cells, and effector lymphocyte T cells following antigenic and inflammatory triggers. The IFN γ dimer binds to its cognate receptor which has two subunits: IFN-γR1 which is the ligand-binding chain (α chain) and IFN-γR2, the signal-transducing chain (β chain). Binding to the receptor activates the JAK/STAT pathway which in turn activates IFN γ responsive genes. While IFN γ can inhibit viral replication, it also works as an immune-modulator and immune-stimulator by increasing surface expression of class I MHC proteins (PMID: 19268625; 10688427)

Synonyms

IFG, IFI, IFN gamma, IFNG, Immune interferon, Interferon gamma, interferon, gamma

Publications

Species	Title
mouse	EMBO Mol Med; Glutathione peroxidase 8 negatively regulates caspase-4/11 to protect against colitis.; Authors - Jye-Lin Hsu
human	Virulence; The lipoprotein NlpD in Cronobacter sakazakii responds to acid stress and regulates macrophage resistance and virulence by maintaining membrane integrity.; Authors - Xuemeng Ji
human	Cancer Sci; CPI-203 improves the efficacy of anti-PD-1 therapy by inhibiting the induced PD-L1 overexpression in liver cancer.; Authors - Xiaoge Niu
human	Clin Immunol; Therapeutic polyclonal human CD8+ CD25+ Fox3+ TNFR2+ PD-L1+ regulatory cells induced ex-vivo.; Authors - David AHorwitz
mouse	FEBS Lett; Linear ubiquitination-induced necrotic tumor remodeling elicits immune evasion; Authors - Katsuhiro Sasaki
human	Int J Mol Med; IRF1 regulates the progression of colorectal cancer via interferon‑induced proteins.; Authors - Xiaohui Xu