HumanKine® recombinant human IFN gamma protein

• HEK293 expressed
• Endotoxin-free
• Animal-component free

Activity(Primary)

0.02-0.14 ng/mL

Specific Activity

minimally 1.00 x 10⁷ IU/mg

Species Reactivity

human,mouse

Purity

>95%

Publications

27

Reviews (1)

Cat no : HZ-1301

Synonyms

IFG, IFI, IFN gamma, IFNG, Immune interferon, Interferon gamma, interferon, gamma

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Validation Data Gallery

• 

  Purity of recombinant human IFN gamma was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue

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  Recombinant human IFN gamma (HZ-1301) dose-dependently inhibits proliferation of the HT-29 human colorectal adenocarcinoma cell line. Cell number was quantitatively assessed by PrestoBlue® cell viability reagent. HT-29 cells were treated with increasing concentrations of recombinant IFN gamma for 72 hours. The EC50 was determined using a 4-parameter non-linear regression model.The EC50 range is 0.02-0.14 ng/mL.

• 

  Proteintech IFN gamma (HZ-1301) demonstrates a 3-fold lower EC50 and equivalent dose-dependent inhibition of proliferation to a leading competitor. Recombinant human IFN gamma dose-dependently inhibits proliferation of the HT-29 human colorectal adenocarcinoma cell line. Cell number was quantitatively assessed by PrestoBlue® cell viability reagent. HT-29 cells were treated with increasing concentrations of recombinant IFN gamma for 72 hours. The EC50 was determined using a 4-parameter non-linear regression model.The EC50 range is 0.02-0.14 ng/mL.

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Technical Specifications

• GeneID: 3458
• Species: Human
• Expression: HEK293
• Activity(Primary): 0.02-0.14 ng/mL
• Specific Activity: minimally 1.00 x 10^7 IU/mg
• Purity: >95%
• Endotoxin: <1 EU/μg
• Accession Number: P01579
• Molecular Mass: 17 and 21 to 32 kDa reduced and non-reduced, homodimer, glycosylated
• Formulation: 1x PBS, See Certificate of Analysis for details
• Species Reactivity: human,mouse

Stability and Reconstitution

Stability and Storage	Product Form	Temperature Conditions	Storage Time (From Date of Receipt)
	Lyophilized	-20°C to -80°C	Until Expiry Date
	Lyophilized	Room Temperature	2 weeks
	Reconstituted as per CofA	-20°C to -80°C	6 months
	Reconstituted as per CofA	4°C	1 week
	Avoid repeated freeze-thaw cycles.

• Reconstitution: Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein to 0.2 mg/mL in sterile 1x PBS pH 7.4 containing 0.1% endotoxin-free recombinant human albumin (HSA). Gently swirl or tap vial to mix.

Background

Interferon gamma (IFN γ), is a type II interferon that provides immunity against bacterial, viral and protozoan infections. In its active form, IFN γ is a glycosylated, non-covalently linked homodimer of 17, 21-32 kDa subunits. It is produced by a number of immune cell types including natural killer cells, natural killer T cells, and effector lymphocyte T cells following antigenic and inflammatory triggers. The IFN γ dimer binds to its cognate receptor which has two subunits: IFN-γR1 which is the ligand-binding chain (α chain) and IFN-γR2, the signal-transducing chain (β chain). Binding to the receptor activates the JAK/STAT pathway which in turn activates IFN γ responsive genes. While IFN γ can inhibit viral replication, it also works as an immune-modulator and immune-stimulator by increasing surface expression of class I MHC proteins (PMID: 19268625; 10688427)

Synonyms

IFG, IFI, IFN gamma, IFNG, Immune interferon, Interferon gamma, interferon, gamma

Publications

Species	Title
human,mouse	Cell Host Microbe; Fiber- and acetate-mediated modulation of MHC-II expression on intestinal epithelium protects from Clostridioides difficile infection; Authors - José L Fachi
mouse	EMBO Mol Med; Glutathione peroxidase 8 negatively regulates caspase-4/11 to protect against colitis.; Authors - Jye-Lin Hsu
human	Virulence; The lipoprotein NlpD in Cronobacter sakazakii responds to acid stress and regulates macrophage resistance and virulence by maintaining membrane integrity.; Authors - Xuemeng Ji
human	Mol Neurobiol; Teriflunomide Promotes Blood-Brain Barrier Integrity by Upregulating Claudin-1 via the Wnt/β-catenin Signaling Pathway in Multiple Sclerosis; Authors - Yipeng Zhao
human	Eur J Pharmacol; Sappanone A ameliorated imiquimod-induced psoriasis-like dermatitis in BALB/c mice via suppressing Mmp8 expression and IL-17 signaling pathway; Authors - Hongyang Li
human	Cancer Sci; CPI-203 improves the efficacy of anti-PD-1 therapy by inhibiting the induced PD-L1 overexpression in liver cancer.; Authors - Xiaoge Niu

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Kathirvel (Verified Customer) (05-21-2024)

I have used this cytokine to induce and activate THP-1 derived macrophages and human MDM to check the immune response of macrophages against Mtb. We observed a good immune primed response from the marophages and it helped to get a good results. specially when we tested Mtb mutant strain to subvert host immune response and this cytokine kind of served as a control to study the immune response.

• Applications: Cell culture
• Cell Tissue Type: THP-1 and MDM