• HEK293 expressed
  • Endotoxin-free
  • Animal-component free

HumanKine® recombinant human IFN gamma protein


0.02-0.14 ng/mL EC50

Specific Activity

>1.00 x 107 IU/mg

Species Reactivity




Cat no : HZ-1301


IFG, IFI, IFN gamma, IFNG, Immune interferon, Interferon gamma, interferon, gamma

Technical Specifications

GeneID 3458
Species Human
Expression HEK293
Activity(Primary) 0.02-0.14 ng/mL EC50
Specific Activity>1.00 x 107 IU/mg
Purity >95%
Endotoxin<1 EU/μg
Accession NumberP01579
Molecular Mass 29-32 kDa
Formulation 1x PBS, See Certificate of Analysis for details
Species Reactivity human,mouse

Stability and Reconstitution

Stability and StorageProduct FormTemperature ConditionsStorage Time
(From Date of Receipt)
Lyophilized-20°C to -80°CUntil Expiry Date
LyophilizedRoom Temperature2 weeks
Reconstituted as per CofA-20°C to -80°C6 months
Reconstituted as per CofA4°C1 week
Avoid repeated freeze-thaw cycles.
Reconstitution Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein in sterile 1x PBS pH 7.4 containing 0.1% endotoxin-free recombinant human albumin (HSA).


Interferon gamma (IFN γ), is a type II interferon that provides immunity against bacterial, viral and protozoan infections. In its active form, IFN γ is a glycosylated, non-covalently linked homodimer of 29-32 kDa subunits. It is produced by a number of immune cell types including natural killer cells, natural killer T cells, and effector lymphocyte T cells following antigenic and inflammatory triggers. The IFN γ dimer binds to its cognate receptor which has two subunits: IFN-γR1 which is the ligand-binding chain (α chain) and IFN-γR2, the signal-transducing chain (β chain). Binding to the receptor activates the JAK/STAT pathway which in turn activates IFN γ responsive genes. While IFN γ can inhibit viral replication, it also works as an immune-modulator and immune-stimulator by increasing surface expression of class I MHC proteins (PMID: 19268625; 10688427)


IFG, IFI, IFN gamma, IFNG, Immune interferon, Interferon gamma, interferon, gamma



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