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Animal-free Recombinant Human IFN alpha 2B0 Publications

Catalog number: HZ-1072

HEK293 expressed Endotoxin-free Animal-component free

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Format: Lyophilized

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Technical Specifications

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IFN alpha 2B activity assay

The activity was determined by the dose-dependent cytotoxicity of the human TF -1 cell line (human erythroleukemic indicator cell line) using the Promega CellTiter96® Aqueous Non-Radioactive Cell Proliferation Assay.

IFN alpha 2B purity

The protein was resolved by SDS-polyacrylamide gel electrophoresis and the gel was stained with Coomassie blue. R represents reducing conditions and NR represents non-reducing conditions.

GeneID:
3440
Species:
Human
Expression:
HEK293
Activity:
Typically ≤0.12 ng/mL EC50
Purity:
>95%
Molecular Mass:
16 kDa, monomer, glycosylated
Formulation
1x PBS, See Certificate of Analysis for details
Species Reactivity:
human

Stability and Reconstitution

Stability and Storage:
Lyophilized proteins are stable for 1 year from the date of receipt if stored between (-20oC) and (-80oC). Upon reconstitution we recommend that the solution can be stored at (4oC) for short term or at (-20oC) to (-80oC) for long term. Repeated freeze thaw cycles should be avoided with reconstituted products.


Reconstitution:
Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein in sterile 1xPBS containing 0.1% endotoxin-free recombinant human serum albumin (HSA).

Product Description

Product Description:

Animal-free Recombinant Human IFN alpha 2B is expressed in human 293 cells as a monomeric glycoprotein with an apparent molecular mass of 16 kDa. This cytokine is produced in a serum-free, chemically defined media. Production in human 293 cells offers authentic glycosylation which contributes to stability in cell growth media and other applications. The purity is greater than 95%.



Background:

Interferon alpha 2 is a type 1 interferon secreted by virus-infected cells to protect surrounding cells from the pathogen. Recombinant human interferon alpha 2 has been approved for therapeutic application in a range of human oncological and viral diseases. Three Interferon alpha 2 subvariants (2A, 2B, 2C) differ by only one or two amino acids at positions 23 and/or 34 of the mature protein (PMID: 25982860; 10672347; 1694761)



Synonyms:
IFNalpha2B



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