HumanKine® recombinant human Activin A protein

• HEK293 expressed
• Endotoxin-free
• Animal-component free

Activity(Primary)

0.5-3.5 ng/mL

Specific Activity

minimally 4.00 x 10² IU/mg

Species Reactivity

human,mouse

Purity

>95%

Publications

29

Reviews (2)

Cat no : HZ-1138

Synonyms

Activin A, Activin beta A chain, EDF, FRP, INHBA, Inhibin beta A chain, inhibin, beta A

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Validation Data Gallery

• 

  Purity of recombinant human Activin A was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

• 

  Recombinant human Activin A (HZ-1138) causes dose-dependent inhibition of proliferation in the Mouse plasmacytoma (MPC-11) cell line. Proliferation of the MPC-11 cell line was assessed using Promega CellTiter 96®. MPC-11 cells were treated with increasing concentrations of recombinant human Activin A for 72 hrs hours before addition of CellTiter96® reagent. The EC50 was determined using a 4-parameter non-linear regression model. The EC50 values range from 0.5-3.5 ng/ml.

• 

  Human induced pluripotent cells(iPSCs) were differentiated to definitive endoderm using HumanKine growth factors (Activin A- HZ-1138, bFGF-HZ-1285, BMP-4- HZ-1045, and Wnt3A-HZ-1296). The differentiation was confirmed by detection of expression of FOXA2(Red) and downregulation of pluripotency marker NANOG(yellow), in immunofluorescence

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Technical Specifications

• GeneID: 3624
• Species: Human
• Expression: HEK293
• Activity(Primary): 0.5-3.5 ng/mL
• Specific Activity: minimally 4.00 x 10^2 IU/mg
• Purity: >95%
• Endotoxin: <1 EU/μg
• Accession Number: P08476
• Molecular Mass: 13kDa reduced, 22 kDa non-reduced, homodimer, non-glycosylated
• Formulation: 1x PBS, See Certificate of Analysis for details
• Species Reactivity: human,mouse

Stability and Reconstitution

Stability and Storage	Product Form	Temperature Conditions	Storage Time (From Date of Receipt)
	Lyophilized	-20°C to -80°C	Until Expiry Date
	Lyophilized	Room Temperature	2 weeks
	Reconstituted as per CofA	-20°C to -80°C	6 months
	Reconstituted as per CofA	4°C	1 week
	Avoid repeated freeze-thaw cycles.

Reconstitution

Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein to 0.2 mg/mL in sterile 1x PBS pH 7.4 containing 0.1% endotoxin-free recombinant human serum albumin (HSA). Gently Swirl or tap vial to mix

Whitepapers

Humankine Activin A has authentic protein maturation and high activity

Background

Activin A, a member of the TGF-β superfamily, was named for its ability to activate FSH secretion from the anterior pituitary gland (PMID 16885530). Three activins have been identified in human tissues: activin A, activin B, and activinAB, of which activin A is the most studied. Activin A has various functions in regulating cell differentiation, proliferation, apoptosis and carcinogenesis (PMID 8200957). Xeno-free Recombinant Human Activin A is expressed from human 293 cells as disulfide linked homodimers with molecular mass of 25 kDa.

Synonyms

Activin A, Activin beta A chain, EDF, FRP, INHBA, Inhibin beta A chain, inhibin, beta A

Publications

Species	Title
human	Cell Res; Visualization of aging-associated chromatin alterations with an engineered TALE system.; Authors - Ruotong Ren
human	Biomater Res; Self-organized insulin-producing β-cells differentiated from human omentum-derived stem cells and their in vivo therapeutic potential; Authors - Ji Hoon Jeong
mouse	EMBO J; HIF1α induced switch from bivalent to exclusively glycolytic metabolism during ESC-to-EpiSC/hESC transition.; Authors - WenyuZhou
human,mouse	Proc Natl Acad Sci U S A; Derivation of naive human embryonic stem cells.; Authors - Carol BWare
human	Nat Protoc; The generation of kidney organoids by differentiation of human pluripotent cells to ureteric bud progenitor-like cells.; Authors - Yun Xia
human	Protein Cell; CRISPR/Cas9-mediated gene knockout reveals a guardian role of NF-κB/RelA in maintaining the homeostasis of human vascular cells.; Authors - Ping Wang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Enes Yagiz (Verified Customer) (08-23-2022)

It is able differentiate human embryonic stemcells into primitive endoderm

• Applications: Cell culture
• Cell Tissue Type: hESCs

FH

Tobias (Verified Customer) (09-13-2021)

HumanKine Activin A is a great product for stem cells, allowing successful differentiation.

• Applications: Cell culture,Other
• Cell Tissue Type: Stem cell differentiation