HumanKine® recombinant human Activin A protein

• HEK293 expressed
• Endotoxin-free
• Animal-component free

Activity

0.5-3.5 ng/mL EC50

Species Reactivity

human,mouse

Purity

>95%

Publications

24

Cat no : HZ-1138

Synonyms

Activin A, Activin beta A chain, EDF, FRP, INHBA, Inhibin beta A chain, inhibin, beta A

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Validation Data Gallery

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  The protein was resolved by SDS- polyacrylamide gel electrophoresis and the gel was stained with Coomassie blue.

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  Recombinant human Activin A (HZ-1138) causes dose-dependent inhibition of proliferation in the Mouse plasmacytoma (MPC-11) cell line. Proliferation of the MPC-11 cell line was assessed using Promega CellTiter 96®. MPC-11 cells were treated with increasing concentrations of recombinant human Activin A for 72 hrs hours before addition of CellTiter96® reagent. The EC50 was determined using a 4-parameter non-linear regression model. The EC50 values range from 0.5-3.5 ng/ml.

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Technical Specifications

• GeneID: 3624
• Species: Human
• Expression: HEK293
• Activity: 0.5-3.5 ng/mL EC50
• Purity: >95%
• Endotoxin: <1 EU/μg
• Accession Number: P08476
• Molecular Mass: 25 kDa, homodimer, non-glycosylated
• Formulation: 1x PBS, See Certificate of Analysis for details
• Species Reactivity: human,mouse

Stability and Reconstitution

Stability and Storage	Product Form	Temperature Conditions	Storage Time (From Date of Receipt)
	Lyophilized	-20°C to -80°C	Until Expiry Date
	Lyophilized	Room Temperature	2 weeks
	Reconstituted as per CofA	-20°C to -80°C	6 months
	Reconstituted as per CofA	4°C	1 week
	Avoid repeated freeze-thaw cycles.

Reconstitution

Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein in sterile 1xPBS containing 0.1% endotoxin-free recombinant human serum albumin (HSA).

Whitepapers

Humankine Activin A has authentic protein maturation and high activity

Background

Activin A, a member of the TGF-β superfamily, was named for its ability to activate FSH secretion from the anterior pituitary gland (PMID 16885530). Three activins have been identified in human tissues: activin A, activin B, and activinAB, of which activin A is the most studied. Activin A has various functions in regulating cell differentiation, proliferation, apoptosis and carcinogenesis (PMID 8200957). Xeno-free Recombinant Human Activin A is expressed from human 293 cells as disulfide linked homodimers with molecular mass of 25 kDa.

Synonyms

Activin A, Activin beta A chain, EDF, FRP, INHBA, Inhibin beta A chain, inhibin, beta A

Publications

Species	Title
human	Protein Cell; Modeling CADASIL vascular pathologies with patient-derived induced pluripotent stem cells.; Authors - Chen Ling
human	Tissue Eng Part C Methods; Essential components for ex vivo proliferation of mesenchymal stromal cells.; Authors - NatalieFekete
human	Cell Rep; Genome-wide R-loop Landscapes during Cell Differentiation and Reprogramming.; Authors - Pengze Yan
human	STAR Protoc; Protocol for Differentiation of Human iPSCs into Pulmonary Neuroendocrine Cells.; Authors - Pooja Hor
mouse	Birth Defects Res B Dev Reprod Toxicol; Lost in translation: The search for an in vitro screen for spermatogenic toxicity.; Authors - Robert EChapin
human	Eur J Obstet Gynecol Reprod Biol; Effects of gremlin-2 on the transition of primordial follicles during early folliculogenesis in the human ovary.; Authors - YukiIkeda