• HEK293 expressed
  • Endotoxin-free
  • Animal-component free

HumanKine® recombinant human Activin A protein

Activity(Primary)

0.5-3.5 ng/mL EC50

Specific Activity

>4.00 x 102 IU/mg

Species Reactivity

human,mouse

Purity

>95%

Cat no : HZ-1138

Synonyms

Activin A, Activin beta A chain, EDF, FRP, INHBA, Inhibin beta A chain, inhibin, beta A



Technical Specifications

GeneID 3624
Species Human
Expression HEK293
Activity(Primary) 0.5-3.5 ng/mL EC50
Specific Activity>4.00 x 102 IU/mg
Purity >95%
Endotoxin<1 EU/μg
Accession NumberP08476
Molecular Mass 22 kDa, homodimer, non-glycosylated
Formulation 1x PBS, See Certificate of Analysis for details
Species Reactivity human,mouse

Stability and Reconstitution

Stability and StorageProduct FormTemperature ConditionsStorage Time
(From Date of Receipt)
Lyophilized-20°C to -80°CUntil Expiry Date
LyophilizedRoom Temperature2 weeks
Reconstituted as per CofA-20°C to -80°C6 months
Reconstituted as per CofA4°C1 week
Avoid repeated freeze-thaw cycles.
Reconstitution Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein in sterile 1xPBS pH 7.4 containing 0.1% endotoxin-free recombinant human serum albumin (HSA).

Background

Activin A, a member of the TGF-β superfamily, was named for its ability to activate FSH secretion from the anterior pituitary gland (PMID 16885530). Three activins have been identified in human tissues: activin A, activin B, and activinAB, of which activin A is the most studied. Activin A has various functions in regulating cell differentiation, proliferation, apoptosis and carcinogenesis (PMID 8200957). Xeno-free Recombinant Human Activin A is expressed from human 293 cells as disulfide linked homodimers with molecular mass of 25 kDa.

Synonyms

Activin A, Activin beta A chain, EDF, FRP, INHBA, Inhibin beta A chain, inhibin, beta A

Publications

SpeciesTitle
human

Cell Res

Visualization of aging-associated chromatin alterations with an engineered TALE system.

Authors - Ruotong Ren
mouse

EMBO J

HIF1α induced switch from bivalent to exclusively glycolytic metabolism during ESC-to-EpiSC/hESC transition.

Authors - WenyuZhou
human,mouse

Proc Natl Acad Sci U S A

Derivation of naive human embryonic stem cells.

Authors - Carol BWare
human

Nat Protoc

The generation of kidney organoids by differentiation of human pluripotent cells to ureteric bud progenitor-like cells.

Authors - Yun Xia
human

Protein Cell

CRISPR/Cas9-mediated gene knockout reveals a guardian role of NF-κB/RelA in maintaining the homeostasis of human vascular cells.

Authors - Ping Wang
mouse

Stem Cell Reports

Conversion of Fibroblast into Functional Leydig-like Cell Using Defined Small Molecules.

Authors - Yan Yang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Enes Yagiz (Verified Customer) (08-23-2022)

It is able differentiate human embryonic stemcells into primitive endoderm

  • Applications: Cell culture
  • Cell Tissue Type: hESCs
FH

Tobias (Verified Customer) (09-13-2021)

HumanKine Activin A is a great product for stem cells, allowing successful differentiation.

  • Applications: Cell culture,Other
  • Cell Tissue Type: Stem cell differentiation