HumanKine® recombinant human VEGF121 protein- GMP grade

• HEK293 expressed
• Endotoxin-free
• Animal-component free

EC50

1.5-9 ng/mL

Species Reactivity

human,pig

Purity

>95%

Cat no : HZ-1204-GMP

Synonyms

L VEGFA, MVCD1, Vascular permeability factor, VEGF, VEGF A, VEGF121, VEGFA, VPF

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Validation Data Gallery

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  Purity of GMP recombinant human VEGF121 was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

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  GMP Recombinant human GMP VEGF121 (HZ-1204-GMP) induces dose-dependent luciferase production in a HEK293T VEGF luciferase reporter cell line. Luciferase production was assessed by the One-Step™ luciferase assay Kit. HEK293T VEGF luciferase reporter cells were treated with increasing concentrations of GMP recombinant VEGF121 for 18 hours. The EC50 was determined using a 4-parameter non-linear regression model. Activity determination was conducted in triplicate on a validated bioassay. The EC50 range is 1.5-9 ng/mL

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Technical Specifications

• GeneID: 7422
• Species: Human
• Expression: HEK293
• EC50: 1.5-9 ng/mL
• Purity: >95%
• Endotoxin: <0.1 EU/μg
• Accession Number: P15692
• Molecular Mass: 17 and 21 kDa reduced, 28 to 40 kDa non-reduced, homodimer and homotrimer, glycosylated
• Formulation: 1x PBS, See Certificate of Analysis for details
• Species Reactivity: human,pig

Stability and Reconstitution

Stability and Storage	Product Form	Temperature Conditions	Storage Time (From Date of Receipt)
	Lyophilized	-20°C to -80°C	Until Expiry Date
	Lyophilized	Room Temperature	2 weeks
	Reconstituted as per CofA	-20°C to -80°C	6 months
	Reconstituted as per CofA	4°C	1 week
	Avoid repeated freeze-thaw cycles.

Reconstitution

Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein to 0.2 mg/mL in sterile 1x PBS pH 7.4 containing 0.1% endotoxin-free recombinant human serum albumin (HSA). Gently swirl or tap vial to mix.

GMP Quality Policies

HumanKine® GMP (Good Manufacturing Practice) recombinant proteins are manufactured and validated in accordance with ISO 13485 quality management system and is compliant with GMP.
Our GMP recombinant proteins are animal component free (ACF), xeno free (XF) and tag free (TF). Read more about these policies here.

Background

This gene is a member of the PDGF/VEGF growth factor family. It encodes a heparin-binding protein, which exists as a disulphide-linked homodimer. This growth factor induces proliferation and migration of vascular endothelial cells and is essential for physiological and pathological angiogenesis. Misregulation of this gene in mice results in abnormal embryonic blood vessel formation (PMID:10838264; 11329058).

Synonyms

L VEGFA, MVCD1, Vascular permeability factor, VEGF, VEGF A, VEGF121, VEGFA, VPF