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  • HEK293 expressed
  • Endotoxin-free
  • Animal-component free

HumanKine® recombinant human VEGF-C protein- GMP grade

EC50

2-16 ng/mL

Species Reactivity

human

Purity

> 95%

ISO 13485 certified HumanKine® human cell-expressed cytokines and growth factors

Cat no : HZ-1336-GMP

Synonyms

FLT4 ligand, FLT4-L, Vascular endothelial growth factor related protein (VRP), VRP, VEGFC, VRPFLT4 ligand DHM

Validation Data Gallery

  • Purity of recombinant human VEGF-C was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

    Purity of recombinant human VEGF-C was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

  • Recombinant human VEGF-C (HZ-1336) induces dose-dependent luciferase production in a HEK293T reporter cell line. Luciferase assay production was assessed by One-Step™ luciferase assay Kit. HEK293T reporter cells were treated with increasing concentrations of recombinant VEGF-C for 18 hours. The EC50 was determined using a 4-parameter non-linear regression model. Activity determination was conducted in triplicate on a validated bioassay. The EC50 range is 2-16 ng/mL.

    Recombinant human VEGF-C (HZ-1336) induces dose-dependent luciferase production in a HEK293T reporter cell line. Luciferase assay production was assessed by One-Step™ luciferase assay Kit. HEK293T reporter cells were treated with increasing concentrations of recombinant VEGF-C for 18 hours. The EC50 was determined using a 4-parameter non-linear regression model. Activity determination was conducted in triplicate on a validated bioassay. The EC50 range is 2-16 ng/mL.

Technical Specifications

GeneID 7424
Species Human
Expression HEK293
EC50 2-16 ng/mL
Purity > 95%
Endotoxin<0.1 EU/μg
Accession NumberP49767
Molecular Mass 18 and 28 kDa, homodimer, glycosylated
Formulation 50 mM Acetate pH 4.5 + 250 mM NaCl
Species Reactivity human

Stability and Reconstitution

Stability and StorageProduct FormTemperature ConditionsStorage Time
(From Date of Receipt)
Lyophilized-20°C to -80°CUntil Expiry Date
LyophilizedRoom Temperature2 weeks
Reconstituted as per CofA-20°C to -80°C6 months
Reconstituted as per CofA4°C1 week
Avoid repeated freeze-thaw cycles.
Reconstitution Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein at 0.2 mg/mL in sterile 1x PBS pH 7.4 containing 0.1% endotoxin-free recombinant serum albumin (HSA). Gently swirl or tap vial to mix

GMP Quality Policies

HumanKine® GMP (Good Manufacturing Practice) recombinant proteins are manufactured and validated in accordance with ISO 13485 quality management system and is compliant with GMP.
Our GMP recombinant proteins are animal component free (ACF), xeno free (XF) and tag free (TF). Read more about these policies here.

Background

N/A

Synonyms

FLT4 ligand, FLT4-L, Vascular endothelial growth factor related protein (VRP), VRP, VEGFC, VRPFLT4 ligand DHM