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  • HEK293 expressed
  • Endotoxin-free
  • Animal-component free

HumanKine® recombinant human Thrombin protein- GMP grade

EC50

1000-5000 units/mg

Species Reactivity

human

Purity

> 95%

ISO 13485 certified HumanKine® human cell-expressed cytokines and growth factors

Cat no : HZ-3010-GMP

Synonyms

Coagulation factor II, F2, Prothrombin, PT, Thrombin

Validation Data Gallery

  • Purity of recombinant human GMP thrombin was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

    Purity of recombinant human GMP thrombin was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

  • The activity of GMP thrombin (HZ-3010-GMP) was measured by the rate of free chromophore formation from Chromozym TH thrombin substrate monitored by absorbance at 405 nm. The enzymatic reaction was carried out in 50mM Tris-HCl pH 8.3 buffer containing 0.1% BSA and 227 mM NaCl at 25°C. Activity was conducted in triplicate on a validated bioassay. Activity ranges from 1000-5000 units/mg.

    The activity of GMP thrombin (HZ-3010-GMP) was measured by the rate of free chromophore formation from Chromozym TH thrombin substrate monitored by absorbance at 405 nm. The enzymatic reaction was carried out in 50mM Tris-HCl pH 8.3 buffer containing 0.1% BSA and 227 mM NaCl at 25°C. Activity was conducted in triplicate on a validated bioassay. Activity ranges from 1000-5000 units/mg.

Technical Specifications

GeneID 2147
Species Human
Expression HEK293
EC50 1000-5000 units/mg
Purity > 95%
Endotoxin<0.1 EU/μg
Accession NumberP00734
Molecular Mass 34 kDa reduced, 36 kDa non-reduced, monomer, glycosylated
Formulation 20 mM MES pH 6.0 + 500 mM Choline Chloride, See Certificate of Analysis for details
Species Reactivity human

Stability and Reconstitution

Stability and StorageThis protein is stable for 1 year from the date of receipt if stored between (-20°C) and (-80°C). Repeated freeze thaw cycles should be avoided.
Reconstitution Not Applicable. Product provided in liquid format.

GMP Quality Policies

HumanKine® GMP (Good Manufacturing Practice) recombinant proteins are manufactured and validated in accordance with ISO 13485 quality management system and is compliant with GMP.
Our GMP recombinant proteins are animal component free (ACF), xeno free (XF) and tag free (TF). Read more about these policies here.

Background

Coagulation factors are a group of related proteins that are essential for normal blood clotting (haemostasis). After an injury, clots protect the body by sealing off damaged blood vessels and preventing further blood loss. Prothrombin circulates in the bloodstream in an inactive form until an injury occurs. In response to that, prothrombin is converted to its active form, thrombin. Thrombin next converts a protein called fibrinogen into fibrin. Thrombin is also important for cell growth and division, tissue repair, and new blood vessels formation - angiogenesis (PMID: 11154146; 16549895; 15892853; 12421139).

Synonyms

Coagulation factor II, F2, Prothrombin, PT, Thrombin