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  • HEK293 expressed
  • Endotoxin-free
  • Animal-component free

HumanKine® recombinant human IL-34 protein- GMP grade

EC50

0.45-4.5 ng/mL

Species Reactivity

Purity

>95%

ISO 13485 certified HumanKine® human cell-expressed cytokines and growth factors

Cat no : HZ-1316-GMP

Synonyms

IL34, Interleukin-34, C16orf77, MGC34647

Validation Data Gallery

  • Purity of GMP-grade recombinant human IL-34 was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

    Purity of GMP-grade recombinant human IL-34 was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

  • Recombinant human IL-34 induces dose-dependent release of MCP-1 in human peripheral blood mononuclear cells (PBMCs). PBMCs were treated with increasing concentration of IL-34 for 48 hours before supernatant collection. The supernatant was tested for MCP-1 via ELISA kit. The EC50 was determined using a 4-parameter non-linear regression model. Activity determination was conducted in triplicate on a validated bioassay. The EC50 range is 0.45-4.5 ng/mL.

    Recombinant human IL-34 induces dose-dependent release of MCP-1 in human peripheral blood mononuclear cells (PBMCs). PBMCs were treated with increasing concentration of IL-34 for 48 hours before supernatant collection. The supernatant was tested for MCP-1 via ELISA kit. The EC50 was determined using a 4-parameter non-linear regression model. Activity determination was conducted in triplicate on a validated bioassay. The EC50 range is 0.45-4.5 ng/mL.

Technical Specifications

GeneID 146433
Species Human
Expression HEK293
EC50 0.45-4.5 ng/mL
Purity >95%
Endotoxin<0.1 EU/µg
Accession NumberQ6ZMJ4
Molecular Mass 38 kDa reduced, 36 and 68 kDa non-reduced, homodimer, glycosylated
Formulation 1 x PBS, See Certificate of Analysis for details
Species Reactivity

Stability and Reconstitution

Stability and StorageProduct FormTemperature ConditionsStorage Time
(From Date of Receipt)
Lyophilized-20°C to -80°CUntil Expiry Date
LyophilizedRoom Temperature2 weeks
Reconstituted as per CofA-20°C to -80°C6 months
Reconstituted as per CofA4°C1 week
Avoid repeated freeze-thaw cycles.
Reconstitution Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein to 0.2 mg/mL in sterile 1x PBS pH 7.4 + 0.1% HSA. Gently swirl or tap vial to mix.

GMP Quality Policies

HumanKine® GMP (Good Manufacturing Practice) recombinant proteins are manufactured and validated in accordance with ISO 13485 quality management system and is compliant with GMP.
Our GMP recombinant proteins are animal component free (ACF), xeno free (XF) and tag free (TF). Read more about these policies here.

Background

IL-34 is a 6-α-helix, proinflammatory cytokine that serves as a ligand for colony-stimulating factor-1 receptor (CSF1R). It is expressed most abundantly by spleen cells. It primarily stimulates human peripheral blood monocyte proliferation and macrophage colony formation. It is also required for Langerhans cell and microglia development. IL-34 has been shown to play a protective role in cancer and Alzheimer’s disease, but it can promote the progression of several autoimmune, inflammatory, and metabolic diseases. As such IL-34 could be used as a biomarker to mark the progression of these diseases (PMID: 22579672, 27577879, 31940023).

Synonyms

IL34, Interleukin-34, C16orf77, MGC34647