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  • HEK293 expressed
  • Endotoxin-free
  • Animal-component free

HumanKine® recombinant human IL-21 protein

Activity(Primary)

0.25-1.25 ng/mL EC50

Species Reactivity

human

Purity

>95%

Cat no : HZ-1319

Synonyms

Interleukin 21, Interleukin-21, Interleukin-21 isoform, Za11, CVID11

Validation Data Gallery

  • Purity of recombinant human IL-21 was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

    Purity of recombinant human IL-21 was determined by SDS- polyacrylamide gel electrophoresis. The protein was resolved in an SDS- polyacrylamide gel in reducing and non-reducing conditions and stained using Coomassie blue.

  • Recombinant human IL-21 (Cat no: HZ-1319) induces dose-dependent release of IFN-gamma in the NK-92 human cell line. NK-92 cells were treated with increasing concentration of recombinant IL-21 for 72 hours before supernatant collection. The supernatant was tested for IFN gamma using Proteintech’s AuthentiKine™ Human IFN-gamma ELISA Kit (KE00146). The EC50 was determined using a 4-parameter non-linear regression model. The EC50 range in 0.25-1.25 ng/mL.

    Recombinant human IL-21 (Cat no: HZ-1319) induces dose-dependent release of IFN-gamma in the NK-92 human cell line. NK-92 cells were treated with increasing concentration of recombinant IL-21 for 72 hours before supernatant collection. The supernatant was tested for IFN gamma using Proteintech’s AuthentiKine™ Human IFN-gamma ELISA Kit (KE00146). The EC50 was determined using a 4-parameter non-linear regression model. The EC50 range in 0.25-1.25 ng/mL.

Technical Specifications

GeneID 59067
Species Human
Expression HEK293
Activity(Primary) 0.25-1.25 ng/mL EC50
Purity >95%
Endotoxin<1 EU/μg
Accession NumberQ9HBE4
Molecular Mass 18 kDa, monomer, glycosylated
Formulation 1x PBS, See Certificate of Analysis for details
Species Reactivity human

Stability and Reconstitution

Stability and StorageProduct FormTemperature ConditionsStorage Time
(From Date of Receipt)
Lyophilized-20°C to -80°CUntil Expiry Date
LyophilizedRoom Temperature2 weeks
Reconstituted as per CofA-20°C to -80°C6 months
Reconstituted as per CofA4°C1 week
Avoid repeated freeze-thaw cycles.
Reconstitution Briefly centrifuge the vial before opening. It is recommended to reconstitute the protein in sterile 1x PBS pH 7.4 containing 0.1% endotoxin-free recombinant human serum albumin (HSA).

Background

IL-21 is a type I, 4-α-helix bundle cytokine primarily produced by CD4+ T cells and NK cells. It is a strong activator of proliferation and antibody production in B cells but does signal to numerous other immune cells as well. Elevated IL-21 signaling can promote several autoimmune diseases including type I diabetes, systemic lupus erythematosus (SLE), and rheumatoid arthritis (RA). However, it has also been shown to have potent antitumoral activity, making it a promising candidate for immunotherapy and as an adjuvant material in CAR-T cell therapy (PMID: 22235123, 26966515, 27577879).

Synonyms

Interleukin 21, Interleukin-21, Interleukin-21 isoform, Za11, CVID11