Phospho-RCC1 (Ser11) Recombinant antibody, PBS Only

Phospho-RCC1 (Ser11) Uni-rAb^TM Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

230056B10

Cat no : 83630-1-PBS

Synonyms

phospho-RCC1(Ser11), RCC1, RCC1 I, CHC1, 230056B10

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Validation Data Gallery

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  WB analysis using 83630-1-RR (same clone as 83630-1-PBS)

  Non-treated and nocodazole treated HeLa cells were subjected to SDS PAGE followed by western blot with 83630-1-RR (phospho-RCC1(Ser11) antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH (60004-1-Ig) subsequently. This data was developed using the same antibody clone with 83630-1-PBS in a different storage buffer formulation.

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Product Information

83630-1-PBS targets Phospho-RCC1 (Ser11) in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Peptide
• Full Name: regulator of chromosome condensation 1
• Calculated Molecular Weight: 48 kDa
• Observed Molecular Weight: 45-50 kDa
• GenBank Accession Number: NM_001048194
• Gene Symbol: RCC1
• Gene ID (NCBI): 1104
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

CHC1, also named as RCC1, promotes the exchange of ran-bound gdp by gtp. It is involved in the regulation of onset of chromosome condensation in the S-phase. Phosphorylation of RCC1 on serines located in or near its nuclear localization signal activates RCC1 to generate RanGTP on mitotic chromosomes, which is required for spindle assembly and chromosome segregation. This antibody is a rabbit polyclonal antibody raised against human phospho-CHC1(Ser11) antigen.