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Phospho-RCC1 (Ser11) Recombinant antibody

Phospho-RCC1 (Ser11) Uni-rAbTM Recombinant Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

230056B10

Cat no : 83630-1-RR

Synonyms

RCC1, RCC1 I, CHC1, 230056B10

Validation Data Gallery

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  • Western Blot (WB) analysis of various lysates using Phospho-RCC1 (Ser11) Recombinant antibody (83630-1-RR)

    WB analysis using 83630-1-RR

    Non-treated and nocodazole treated HeLa cells were subjected to SDS PAGE followed by western blot with 83630-1-RR (phospho-RCC1(Ser11) antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH (60004-1-Ig) subsequently.

Tested Applications

Positive WB detected inHeLa cells, nocodazole treated HeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

83630-1-RR targets Phospho-RCC1 (Ser11) in WB, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Host / Isotype Rabbit / IgG
Class Recombinant
Type Antibody
Immunogen Peptide
Full Name regulator of chromosome condensation 1
Calculated Molecular Weight 48 kDa
Observed Molecular Weight 45-50 kDa
GenBank Accession NumberNM_001048194
Gene Symbol RCC1
Gene ID (NCBI) 1104
Conjugate Unconjugated
Form Liquid
Purification MethodProtein A purfication
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

CHC1, also named as RCC1, promotes the exchange of ran-bound gdp by gtp. It is involved in the regulation of onset of chromosome condensation in the S-phase. Phosphorylation of RCC1 on serines located in or near its nuclear localization signal activates RCC1 to generate RanGTP on mitotic chromosomes, which is required for spindle assembly and chromosome segregation. This antibody is a rabbit polyclonal antibody raised against human phospho-CHC1(Ser11) antigen.

Protocols

Product Specific Protocols
WB protocol for Phospho-RCC1 (Ser11) antibody 83630-1-RRDownload protocol
Standard Protocols
Click here to view our Standard Protocols