Phospho-TDP43 (Ser403/404) Monoclonal antibody
Phospho-TDP43 (Ser403/404) Monoclonal Antibody for FC, WB, ELISA
Cat no : 66079-1-Ig
|Positive WB detected in||HEK-293 cells, Jurkat cells, K-562 cells, RAW 264.7 cells, fetal human brain tissue|
|Positive FC detected in||Jurkat cells|
|Western Blot (WB)||WB : 1:5000-1:50000|
|Sample-dependent, check data in validation data gallery|
66079-1-Ig targets Phospho-TDP43 (Ser403/404) in WB, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse samples.
|Tested Reactivity||human, mouse|
|Cited Reactivity||human, zebrafish|
|Host / Isotype||Mouse / IgG2a|
|Full Name||TAR DNA binding protein|
|Calculated molecular weight||43 kDa|
|Observed molecular weight||25 kDa|
|GenBank accession number||NM_007375|
|Gene ID (NCBI)||23435|
|Purification Method||Protein A purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.|
Transactivation response (TAR) DNA-binding protein of 43 kDa (also known as TARDBP or TDP-43) was first isolated as a transcriptional inactivator binding to the TAR DNA element of the HIV-1 virus. Neumann et al. (2006) found that a hyperphosphorylated, ubiquitinated, and cleaved form of TARDBP, known as pathologic TDP-43, is the major component of the tau-negative and ubiquitin-positive inclusions that characterize amyotrophic lateral sclerosis (ALS) and the most common pathological subtype of frontotemporal lobar degeneration (FTLD-U). Various forms of TDP-43 exist, including 18-35 kDa of cleaved C-terminal fragments, 45-50 kDa phospho-protein, 55 kDa glycosylated form, 75 kDa hyperphosphorylated form, and 90-300 kDa cross-linked form. (PMID: 17023659,19823856, 21666678, 22193176).66079-1-Ig is a mouse monoclonal antibody recognizing TDP-43 only when phosphorylated at 403/404. Immunohistochemical analyses using this antibody only stain the insoluble inclusions in pathologic tissues without normal diffuse nuclear staining.
TDP-43 condensates and lipid droplets regulate the reactivity of microglia and regeneration after traumatic brain injury
Acta Neuropathol Commun
Influence of APOE genotype in primary age-related tauopathy.
Eur J Med Chem
Targeting nuclear protein TDP-43 by cell division cycle kinase 7 inhibitors: A new therapeutic approach for amyotrophic lateral sclerosis.
Using Tetracysteine-Tagged TDP-43 with a Biarsenical Dye To Monitor Real-Time Trafficking in a Cell Model of Amyotrophic Lateral Sclerosis.
Drosophila lines with mutant and wild type human TDP-43 replacing the endogenous gene reveals phosphorylation and ubiquitination in mutant lines in the absence of viability or lifespan defects.
Methods Mol Biol
Sedimentation Assays to Assess the Impact of Posttranslational Modifications on Phase Separation of RNA-Binding Proteins In Vitro and In Cells
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Azita (Verified Customer) (06-17-2021)
Immunocytochemistry labelling of (4% PFA) fixed NSC-34 cells by Phospho-TDP43 (Ser403/404) Monoclonal antibody at dilution of 1:100 showed strong labelling.