Phospho-TDP43 (Ser403/404) Monoclonal antibody, PBS Only

Phospho-TDP43 (Ser403/404) Monoclonal Antibody for WB, Indirect ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

human, mouse

Applications

WB, Indirect ELISA

Conjugate

Unconjugated

CloneNo.

6B11B12

Cat no : 66079-1-PBS

Synonyms

ALS10, Phospho-TDP43 (Ser403/404), TAR DNA binding protein, TAR DNA binding protein 43, TARDBP, TDP 43, TDP43, tdp-43

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Validation Data Gallery

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  WB analysis using 66079-1-Ig (same clone as 66079-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66079-1-Ig (Phospho-TDP43 (Ser403/404) antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 66079-1-PBS in a different storage buffer formulation.

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  WB analysis of K-562 using 66079-1-Ig (same clone as 66079-1-PBS)

  K-562 cells were subjected to SDS PAGE followed by western blot with 66079-1-Ig (Phospho-TDP43 (Ser403/404) Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66079-1-PBS in a different storage buffer formulation.

• 

  WB analysis of RAW 264.7 using 66079-1-Ig (same clone as 66079-1-PBS)

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 66079-1-Ig (Phospho-TDP43 (Ser403/404) Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66079-1-PBS in a different storage buffer formulation.

• 

  WB analysis of fetal human brain using 66079-1-Ig (same clone as 66079-1-PBS)

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 66079-1-Ig (Phospho-TDP43 (Ser403/404) Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66079-1-PBS in a different storage buffer formulation.

• 

  WB analysis of Jurkat using 66079-1-Ig (same clone as 66079-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 66079-1-Ig (Phospho-TDP43 (Ser403/404) Antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66079-1-PBS in a different storage buffer formulation.

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Product Information

66079-1-PBS targets Phospho-TDP43 (Ser403/404) in WB, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: TAR DNA binding protein
• Calculated Molecular Weight: 43 kDa
• Observed Molecular Weight: 25 kDa
• GenBank Accession Number: NM_007375
• Gene Symbol: TDP-43
• Gene ID (NCBI): 23435
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS Only
• Storage Conditions: Store at -80°C.

Background Information

Transactivation response (TAR) DNA-binding protein of 43 kDa (also known as TARDBP or TDP-43) was first isolated as a transcriptional inactivator binding to the TAR DNA element of the HIV-1 virus. Neumann et al. (2006) found that a hyperphosphorylated, ubiquitinated, and cleaved form of TARDBP, known as pathologic TDP-43, is the major component of the tau-negative and ubiquitin-positive inclusions that characterize amyotrophic lateral sclerosis (ALS) and the most common pathological subtype of frontotemporal lobar degeneration (FTLD-U). Various forms of TDP-43 exist, including 18-35 kDa of cleaved C-terminal fragments, 45-50 kDa phospho-protein, 55 kDa glycosylated form, 75 kDa hyperphosphorylated form, and 90-300 kDa cross-linked form. (PMID: 17023659,19823856, 21666678, 22193176).66079-1-Ig is a mouse monoclonal antibody recognizing TDP-43 only when phosphorylated at 403/404. Immunohistochemical analyses using this antibody only stain the insoluble inclusions in pathologic tissues without normal diffuse nuclear staining.